EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    3.1.8.1 | -999 |
- |
- |
663698, 663774, 664771, 666809, 678836 |
| 3.1.8.1 | -999 |
- |
activity in wild-type strain WCP904 and of purified recombinant enzyme with different organophosphorus pesticides, overview |
749640 |
| 3.1.8.1 | -999 |
- |
assay method evaluation of analogues with fluorescent leaving groups for screening and selection of the enzyme that efficiently hydrolyzes organophosphorus nerve agents, overview |
665987 |
| 3.1.8.1 | -999 |
- |
enzyme activity in individuals of both sexes and different age, overview |
677570 |
| 3.1.8.1 | -999 |
- |
His6-tagged organophosphate hydrolase fused with alpha-helical leucine zipper domains and unstructured soluble linker domains shows specific activities from 1.6-2.2 units for paraoxon, 0.61-1.3 units for parathion and 0.011-0.036 units for ethyl parathion (one unit is defined as mol of substrate per mol enzyme per second), in 50 mM carbonate buffer (pH 10.5), at 25°C |
716817 |
| 3.1.8.1 | -999 |
- |
new enzymatic tests are developed that detect total PON1 levels, irrespective of high-density lipoprotein status and R/Q polymorphism, as well as the degree of catalytic stimulation and increased stability that follows tight binding of PON1 to HDLapoA-I. The tests are based on measuring total PON1 levels with a fluorogenic phosphotriester, measuring the lipolactonase activity with a chromogenic lactone, and assaying the chelator-mediated inactivation rate of the enzyme. The latter two are affected by tight binding of high-density lipoprotein and thereby derive the levels of the serum PON1-HDL complex. The new tests are demonstrated with a group of healthy individuals and show that the levels of PON1-HDL vary by a factor of 12 |
681264 |
| 3.1.8.1 | -999 |
- |
relative rates of hydrolysis for several substrates |
646504 |
| 3.1.8.1 | -999 |
- |
specific activity of five enzyme fractions determined with paraoxon, diazoxon or chlorpyrifos-oxon |
35192 |
| 3.1.8.1 | 0.000008 |
- |
wild type enzyme, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C |
715315 |
| 3.1.8.1 | 0.00015 |
- |
wild type enzyme, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C |
715315 |
