Information on EC 2.7.7.7 - DNA-directed DNA polymerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.7
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RECOMMENDED NAME
GeneOntology No.
DNA-directed DNA polymerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
deoxynucleoside triphosphate + DNAn = diphosphate + DNAn+1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Purine metabolism
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Pyrimidine metabolism
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SYSTEMATIC NAME
IUBMB Comments
deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed)
Catalyses DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. Requires a primer, which may be DNA or RNA. See also EC 2.7.7.49 RNA-directed DNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-90-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Aeropyrum pernix DSM 11879
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain AR39
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Manually annotated by BRENDA team
strain AR39
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Manually annotated by BRENDA team
monkey
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Manually annotated by BRENDA team
Quail
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain R1
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
enzyme overexpressed in Escherichia coli
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Manually annotated by BRENDA team
strain JH39
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Manually annotated by BRENDA team
K12
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Manually annotated by BRENDA team
Escherichia coli infected with
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
isolated from hot springs in Gönen and Hisaralan, Turkey, gene polA
UniProt
Manually annotated by BRENDA team
isolated from hot springs in Gönen and Hisaralan, Turkey, gene polA
UniProt
Manually annotated by BRENDA team
ATCC12980D-5
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Manually annotated by BRENDA team
Herpes simplex virus
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
synonym: Micrococcus lysodeikticus
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Manually annotated by BRENDA team
river loach
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Manually annotated by BRENDA team
two family Y DNA polymerases Rv1537 and Rv3056
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Philothamnus punctatus
green shrub snake
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Manually annotated by BRENDA team
identical to strain KT2440, gene dinB
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
common frog
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Manually annotated by BRENDA team
Ruellia sp.
petunia, Mitchell
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Manually annotated by BRENDA team
gene MIP1 encoding DNA pol gamma, and gene POL1 encoding DNA polymerase alpha catalytic subunit A
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
sea urchin
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Manually annotated by BRENDA team
sea urchin
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Testudines agrionemys
Horsfield’s terrapin
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain DSM 15227
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain ATCC BAA-394, DSM 14981
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain INValphaF' of Escherichia coli transformed with the pTaq plasmid containing the Taq gene expressed under control of the tac promoter
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Manually annotated by BRENDA team
strain YT1
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Manually annotated by BRENDA team
strain GH24
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Manually annotated by BRENDA team
strain GH24
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Manually annotated by BRENDA team
strain Z05
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Manually annotated by BRENDA team
strain Z05
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
isolated from a hot spring at Kagoshima, Japan
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
DNA polymerase A, a gamma-like DNA polymerase
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Manually annotated by BRENDA team
crested newt
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
5 cellular DNA template-dependent DNA polymerases are encoded by distinct genes: polymerase alpha, i.e. pol I, polymerase beta, only in vertebrates, polymerase gamma, required for mitochondrial DNA replication but encoded in the nucleus, polymerase delta, enzymes in mammalian cell contain tightly associated 3'-5'-exonuclease activities, 2 forms: proliferating cell nuclear antigen-dependent and a proliferating cell nuclear antigen-independent, also called DNA polymerase delta II, now named DNA polymerase epsilon, polymerase epsilon, tightly associated 3'-5'-exonuclease activity, formerly named DNA polymerase delta II
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyguanosine triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
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?
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
2-hydroxy-2'-deoxyadenosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
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DNA polymerase eta incorporates 2-hydroxy-2'-deoxyadenosine 5'-triphosphate opposite template G during DNA synthesis
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?
2-thio-dCTP + DNAn
?
show the reaction diagram
5-methyl-dCTP + DNAn
?
show the reaction diagram
7-deaza-2'-deoxyadenosine 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
8-bromo-dATP + DNAn
?
show the reaction diagram
8-hydroxy-2'-deoxyguanosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
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DNA polymerase eta incorporates 8-hydroxy-2'-deoxyguanosine 5'-triphosphate opposite template A and slightly opposite template C during DNA synthesis
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?
8-oxo-dATP + DNAn
?
show the reaction diagram
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dCTP and 5-methyl-dCTP are efficiently incorporated opposite a template guanine but significantly less so opposite a template O6-methylguanine. 2-thio-dCTP is efficiently inserted opposite guanine and is also incorporated opposite O6-methylguanine, to a similar extent as dCTP. Of the dNTPs assayed, dCTP, 5-Me-dCTP, and 2-thio-dCTP display the highest incorporation efficiency opposite O6-methylguanine. dTTP incorporation is favored opposite O6-methylguanine rather than opposite guanine. Hydrophobicity of the incoming dNTP appears to have little influence on the process of nucleotide selection by Dpo4, with hydrogen bonding capacity being a major influence. 8-oxo-dATP and 8-bromo-dATP are not inserted opposite O6-methylguanine and are slowly incorporated opposite guanine. dPTP (i.e. 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-b-d-2’-deoxyribofuranosid-5’-triphosphate) is incorporated opposite guanine slightly less efficiently than dCTP and is not incorporated opposite O6-methylguanine
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?
dATP + DNAn
?
show the reaction diagram
dATP + DNAn
diphosphate + ?
show the reaction diagram
dATP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dCTP + DNAn
?
show the reaction diagram
dCTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
deoxxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
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the phosphoryl transfer step may be rate limiting for the non-cognate nucleotide incorporation by the enzyme
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?
deoxynucleoside triphosphate + DNAn
?
show the reaction diagram
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
deoxynucleoside triphosphate + primed M13n
diphosphate + primed M13n+1
show the reaction diagram
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dGTP + DNAn
?
show the reaction diagram
dGTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dITP + DNAn
?
show the reaction diagram
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?
DNA 21/41-mer + dTTP
? + diphosphate
show the reaction diagram
dPTP + DNAn
?
show the reaction diagram
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i.e. 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-beta-D-2'-deoxyribofuranosid 5'-triphosphate. dCTP and 5-methyl-dCTP are efficiently incorporated opposite a template guanine but significantly less so opposite a template O6-methylguanine. 2-thio-dCTP is efficiently inserted opposite guanine and is also incorporated opposite O6-methylguanine, to a similar extent as dCTP. Of the dNTPs assayed, dCTP, 5-Me-dCTP, and 2-thio-dCTP display the highest incorporation efficiency opposite O6-methylguanine. dTTP incorporation is favored opposite O6-methylguanine rather than opposite guanine. Hydrophobicity of the incoming dNTP appears to have little influence on the process of nucleotide selection by Dpo4, with hydrogen bonding capacity being a major influence. 8-oxo-dATP and 8-bromo-dATP are not inserted opposite O6-methylguanine and are slowly incorporated opposite guanine
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?
dTTP + DNAn
?
show the reaction diagram
dTTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dTTP+ DNAn
?
show the reaction diagram
N1-methyl-2'-deoxyadenosine 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
North-methanocarba-dATP + DNAn
?
show the reaction diagram
poly(dA)/oligo(dT)x + n dTTP
poly(dA)/oligo(dT)x+n + n diphosphate
show the reaction diagram
P0CL76, Q9V2F3 and Q9V2F4
preferred substrate
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?
rATP + DNAn
?
show the reaction diagram
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?
rCTP + DNAn
?
show the reaction diagram
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?
South-methanocarba-dATP + DNAn
?
show the reaction diagram
additional information
?
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