Application | Comment | Organism |
---|---|---|
molecular biology | long and accurate PCR can be achieved with a mixture of wild type DNA polymerase from Thermococcus kodakaraensis and its exonuclease deficient mutant enzyme N210D is utilized (at the ratio of 1:40) | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
N210D | the mutant enzyme shows very weak 3'-5'-exonuclease activity compared to the wild-type enzyme (0.1%). No significant difference in DNA polymerase activity as compared with that of the wild-type enzyme. Mutation frequency in PCR becomes higher as 3'5' exonuclease activity decreases | Thermococcus kodakarensis |
Y311F | the mutant enzyme shows very weak 3'-5'-exonuclease activity compared to the wild-type enzyme (0.01%). No significant difference in DNA polymerase activity as compared with that of the wild-type enzyme | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | P77933 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the DNA polymerase also shows 3'-5' exonuclease activity. The 3'5' exonuclease activity of the DNA polymerase is important for DNA elongation with high fidelity | Thermococcus kodakarensis | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
3'-5' exonuclease assay at | Thermococcus kodakarensis |
75 | - |
DNA polymerase assay at | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
DNA polymerase assay at | Thermococcus kodakarensis |
8 | - |
3'-5' exonuclease assay at | Thermococcus kodakarensis |