Literature summary for 2.7.7.7 extracted from

Fiala, K.A.; Brown, J.A.; Ling, H.; Kshetry, A.K.; Zhang, J.; Taylor, J.S.; Yang, W.; Suo, Z.
Mechanism of template-independent nucleotide incorporation catalyzed by a template-dependent DNA polymerase (2007), J. Mol. Biol., 365, 590-602.

Search for more literature for 2.7.7.7

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of ternary complex Dpo4/blunt-end DNA/dATP, crystals are produced by the hanging-drop method at 20°C	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	mechanism of template-independent nucleotide incorporation. Based on the efficiency ratios, Dpo4 selects nucleotides for blunt-end addition in the order of decreasing efficiency: dATP, dTTP, dCTP, dGTP, with dATP favored by five to 50fold over the other nucleotides. The first bluntend dATP incorporation is 80fold more efficient than the second, and among natural deoxynucleotides, dATP is the preferred substrate due to its stronger intrahelical base-stacking ability	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	mechanism of template-independent nucleotide incorporation. Based on the efficiency ratios, Dpo4 selects nucleotides for blunt-end addition in the order of decreasing efficiency: dATP, dTTP, dCTP, dGTP, with dATP favored by five to 50fold over the other nucleotides. The first bluntend dATP incorporation is 80fold more efficient than the second, and among natural deoxynucleotides, dATP is the preferred substrate due to its stronger intrahelical base-stacking ability	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
Dpo4	-	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)