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Literature summary for 2.7.7.7 extracted from

  • Luo, N.; Kaguni, L.S.
    Mutations in the spacer region of Drosophila mitochondrial DNA polymerase affect DNA binding, processivity, and the balance between Pol and Exo function (2005), J. Biol. Chem., 280, 2491-2497.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
mutant proteins expressed from baculovirus constructs in insect cells Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
DELTA413–470 mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity Drosophila melanogaster
DELTA483–533 expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant holoenzyme is reduced greatly as compared to wild type Drosophila melanogaster
DELTA536–581 expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant enzyme is 80% of wild-type holoenzyme Drosophila melanogaster
DELTA666–742 mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity Drosophila melanogaster
F576A mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity Drosophila melanogaster
G575A mutation in alpha-subunit, mutant enzyme has nearly normal activity Drosophila melanogaster
G575A/W576A/F578A mutation in alpha-subunit completely reduces DNA polymerase activity Drosophila melanogaster
K557A mutation in alpha-subunit, mutant enzyme has nearly normal activity Drosophila melanogaster
K687A/D688A/F689A mutation in alpha-subunit resulted in DNA polymerase activities that is 60–80% of wild-type enzyme Drosophila melanogaster
L558A mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity Drosophila melanogaster
P556A mutation in alpha-subunit, mutant enzyme has nearly normal activity Drosophila melanogaster
P556A/K557A/L558A mutation in alpha-subunit completely reduces DNA polymerase activity Drosophila melanogaster
S719A/Y720A/W721 mutation in alpha-subunit completely reduces DNA polymerase activity Drosophila melanogaster
W576A mutation in alpha-subunit, mutant enzyme is nearly inactive Drosophila melanogaster
Y419A/E420A/D421A mutation in alpha-subunit resulted in DNA polymerase activities that is 60–80% of wild-type enzyme Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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 Drosophila melanogaster 5739
-

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant proteins Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Drosophila melanogaster diphosphate + DNAn+1
-
 ?