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Literature summary for 2.7.7.7 extracted from

  • Lebedeva, N.A.; Rechkunova, N.I.; Dezhurov, S.V.; Degtyarev, S.K.; Lavrik, O.I.
    Photoreactive dTTP analogues as substrates for thermostable DNA polymerase from Thermus thermophilus B35 (2004), Russ. J. Bioorg. Chem., 30, 332-336.
No PubMed abstract available
Search for more literature for 2.7.7.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate pH 9.0, 25°C Thermus thermophilus
0.002
-
 dTTP pH 9.0, 25°C Thermus thermophilus
0.0055
-
 5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate pH 9.0, 25°C Thermus thermophilus
0.04
-
 5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate pH 9.0, 25°C Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus B35
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate + DNAn
-
 Thermus thermophilus ?
-
 ?
5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate + DNAn
-
 Thermus thermophilus B35 ?
-
 ?
5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate + DNAn
-
 Thermus thermophilus ?
-
 ?
5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate + DNAn
-
 Thermus thermophilus B35 ?
-
 ?
5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate + DNAn
-
 Thermus thermophilus ?
-
 ?
5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate + DNAn
-
 Thermus thermophilus B35 ?
-
 ?
deoxynucleoside triphosphate + DNAn several dTTP analoges bearing a photoreactive 2-nitro-5-azidobenzoyl group attached at position 5 of uracil through linkers of various lengths, are substrates in the elongation reaction of the 5'-32P-labeled primer-template complex. The incorporation of the analogs into the 3' primer end did not impede further elongation of the chain in the presence of natural dNTP Thermus thermophilus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn several dTTP analoges bearing a photoreactive 2-nitro-5-azidobenzoyl group attached at position 5 of uracil through linkers of various lengths, are substrates in the elongation reaction of the 5'-32P-labeled primer-template complex. The incorporation of the analogs into the 3' primer end did not impede further elongation of the chain in the presence of natural dNTP Thermus thermophilus B35 diphosphate + DNAn+1
-
 ?
dTTP + DNAn
-
 Thermus thermophilus diphosphate + DNAn+1
-
 ?
dTTP + DNAn
-
 Thermus thermophilus B35 diphosphate + DNAn+1
-
 ?