Literature summary for 2.7.7.7 extracted from

Irimia, A.; Zang, H.; Loukachevitch, L.V.; Eoff, R.L.; Guengerich, F.P.; Egli, M.
Calcium is a cofactor of polymerization but inhibits pyrophosphorolysis by the Sulfolobus solfataricus DNA polymerase Dpo4 (2006), Biochemistry, 45, 5949-5956.

Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+	Saccharolobus solfataricus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	Ca2+ (but not Ba2+, Co2+, Cu2+, Ni2+, or Zn2+) is a cofactor for Dpo4-catalyzed polymerization with both native and 8-oxoG-containing DNA templates. Both dNTP and ddNTP are substrates of the polymerase in the presence of either Mg2+ or Ca2+. No pyrophosphorolysis occurs in the presence of Ca2+	Saccharolobus solfataricus
Mg2+	cofactor for both the polymerase and pyrophosphorolysis activities	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Synonyms

Synonyms	Comment	Organism
DNA polymerase Dpo4	-	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+	Saccharolobus solfataricus

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Release 2023.1 (January 2023)