Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as maltose-binding protein fusion protein | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | parasites overexpressing Tcpolbeta shows reduced levels of 7,8-dihydro-8-oxoguanine in kDNA and an increased survival after treatment with hydrogen peroxide when compared to control cells. The resistance is lost after treating Tcpolbeta overexpressors with methoxiamine, a potent base-excision repair inhibitor | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-azido-2',3'-dideoxythymidine triphosphate | - |
Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetoplast | Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes | Trypanosoma cruzi | 20023 | - |
mitochondrial matrix | - |
Trypanosoma cruzi | 5759 | - |
additional information | subcellular localization of Tcpolbeta is restricted to antipodal sites in replicating cells, while in nonreplicative cultured trypomastigotes Tcpolbeta is dispersed inside its single ramified mitochondrion. The subcellular localization of Tcpolbeta follows the parasite cell cycle, overview | Trypanosoma cruzi | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Trypanosoma cruzi | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes | Trypanosoma cruzi | - |
epimastigote | Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes | Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Trypanosoma cruzi | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | - |
Trypanosoma cruzi | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase beta | - |
Trypanosoma cruzi |
polbeta | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assya at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
physiological function | DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions, Tcpolbeta is involved in oxidative stress response, Tcpolbeta overexpression improves epimastigote survival in presence of H2O2 | Trypanosoma cruzi |