Activating Compound | Comment | Organism | Structure |
---|---|---|---|
proliferating cell nuclear antigen | PCNA, the interaction between pol delta and PCNA is mediated predominantly by the p50 accessory subunit of pol delta and not the p125 catalytic subunit | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L606G | site-directed mutagenesis, the mutant shows increased polymerase activity and slightly reduced exonuclease activity compared to the wild-type enzyme, mutant pol delta L606G is highly error prone, incorporating single noncomplementary nucleotides at a high frequency during DNA synthesis | Homo sapiens |
L606K | site-directed mutagenesis, the mutant shows increased polymerase activity and reduced exonuclease activity compared to the wild-type enzyme, mutant pol delta L606K is extremely accurate, with a higher fidelity of single nucleotide incorporation by the active site than that of wild-type pol delta, it does not catalyze detectable nucleotide mis-insertion even with nucleotide concentrations as high as 4 mM, but pol delta L606K mutant is impaired in the bypass of DNA adducts | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics and single nucleotide kinetics of wild-type and mutat enzymes, overview | Homo sapiens | |
0.00249 | - |
dCTP | mutant L606K, pH 7.5, 37°C, misincorporation of dTTP with G at first position | Homo sapiens | |
0.00464 | - |
dCTP | wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position | Homo sapiens | |
0.00792 | - |
dCTP | mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position | Homo sapiens | |
0.175 | - |
dTTP | wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position | Homo sapiens | |
0.211 | - |
dTTP | mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Homo sapiens | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + DNAn | - |
Homo sapiens | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Homo sapiens | diphosphate + DNAn+1 | - |
? | |
dTTP + DNAn | activity assay with plus-strand m13 DNA annealed to 5'-32P end-labeled primer 5'-GCTGTTGGGAAGGGCGATCG-3' | Homo sapiens | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase delta | - |
Homo sapiens |
pol delta | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.9 | assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | enhanced fidelity of base selection by a polymerase active site can result in impaired lesion bypass and delayed replication fork progression | Homo sapiens |
physiological function | 3'-> 5'-exonucleolytic proofreading activity | Homo sapiens |