Application | Comment | Organism |
---|---|---|
biotechnology | thermostable polymerase used in PCR | Thermus aquaticus |
molecular biology | thermostable polymerase used in PCR | Thermus aquaticus |
Cloned (Comment) | Organism |
---|---|
polymerase I gene expressed in Escherichia coli | Thermus aquaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Thermus aquaticus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Thermus aquaticus YT1 | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Thermus aquaticus YT1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polymerase I, expressed in Escherichia coli | Thermus aquaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
polymerase I | Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermus aquaticus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Thermus aquaticus YT1 | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Thermus aquaticus |