EC Number |
Activating Compound |
Reference |
---|
2.7.7.7 | (NH4)2SO4 |
optimal concentration of 20 mM, inhibition below and above 20 mM |
722133 |
2.7.7.7 | 2-mercaptoethanol |
- |
643596 |
2.7.7.7 | 2-mercaptoethanol |
required for maximal activity |
643597 |
2.7.7.7 | Aeropyrum pernix proliferating cell nuclear antigen 1 |
three genes encoding proliferating cell nuclear antigen (PCNA)-like sequences are identified in the genome of Aeropyrum pernix. The genes are cloned and expressed in Escherichia coli and the gene products are analyzed. All three proliferating cell nuclear antigen homologs stimulate the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II in Aeropyrum pernix to various extents. Aeropyrum pernix PCNA 3 (ApePCNA3) provides a most remarkable effect on both Pol I and Pol II |
727739 |
2.7.7.7 | Aeropyrum pernix proliferating cell nuclear antigen 3 |
three genes encoding proliferating cell nuclear antigen (PCNA)-like sequences are identified in the genome of Aeropyrum pernix. The genes are cloned and expressed in Escherichia coli and the gene products are analyzed. All three proliferating cell nuclear antigen homologs stimulate the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II in Aeropyrum pernix to various extents. Aeropyrum pernix PCNA 3 (ApePCNA3) provides a most remarkable effect on both Pol I and Pol II |
727739 |
2.7.7.7 | ATP |
considerable enhancement in processivity at 1 mM ATP |
690621 |
2.7.7.7 | Auxiliary subunits |
pol III can repair short gaps created by nuclease in duplex DNA, for efficient replication of the long, single-stranded templates pol III requires auxiliary subunits beta, gamma and delta |
643592 |
2.7.7.7 | bovine serum albumin |
DNA polymerase is stabilized with an approximately 25% increase in enzyme activity |
673383 |
2.7.7.7 | dimethyl sulfoxide |
inhibition of DNA polymerase epsilon, stimulation of DNA polymerase delta |
643639 |
2.7.7.7 | dimethyl sulfoxide |
stimulates DNA polymerase alpha and delta, inhibits human DNA polymerase epsilon |
643608 |