Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D424A | mutation in polymerase active site, the polymerase activity is reduced more than 30fold compared to the wild-type activity | Saccharolobus solfataricus |
D424A/D542A | mutation in polymerase active site, complete inactivation of polymerase activity | Saccharolobus solfataricus |
D542A | mutation in polymerase active site, the polymerase activity is reduced more than 50fold compared to the wild-type activity | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P95979 | - |
- |
Saccharolobus solfataricus P2 | P95979 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dpo3 has an active exonuclease proofreading domain, it shows intrinsic exonuclease activity | Saccharolobus solfataricus | ? | - |
? | |
additional information | Dpo3 has an active exonuclease proofreading domain, it shows intrinsic exonuclease activity | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase III | - |
Saccharolobus solfataricus |
Dpo3 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
polymerization activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
preincubation at 70°C for 55 min minimally reduces polymerase activity | Saccharolobus solfataricus |
94 | - |
estimated melting temperature is avove 94°C, which is increased slightly in the presence of DNA | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the fidelity of nucleotide incorporation by Dpo3 from the polymerase active site alone is 1000-10000 at 37°C. The functional exonuclease proofreading active site will increase fidelity by at least 100 | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | maximal activity is observed with 20 mM Tris between pH 7 and 8.5 | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | Dpo3 is a potential player in the proper maintenance of the archaeal genome | Saccharolobus solfataricus |