Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Bauer, R.J.; Begley, M.T.; Trakselis, M.A.
    Kinetics and fidelity of polymerization by DNA polymerase III from Sulfolobus solfataricus (2012), Biochemistry, 51, 1996-2007.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D424A mutation in polymerase active site, the polymerase activity is reduced more than 30fold compared to the wild-type activity Saccharolobus solfataricus
D424A/D542A mutation in polymerase active site, complete inactivation of polymerase activity Saccharolobus solfataricus
D542A mutation in polymerase active site, the polymerase activity is reduced more than 50fold compared to the wild-type activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P95979
-
-
Saccharolobus solfataricus P2 P95979
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Dpo3 has an active exonuclease proofreading domain, it shows intrinsic exonuclease activity Saccharolobus solfataricus ?
-
 ?
additional information Dpo3 has an active exonuclease proofreading domain, it shows intrinsic exonuclease activity Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase III
-
 Saccharolobus solfataricus
Dpo3
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 polymerization activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 preincubation at 70°C for 55 min minimally reduces polymerase activity Saccharolobus solfataricus
94
-
 estimated melting temperature is avove 94°C, which is increased slightly in the presence of DNA Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the fidelity of nucleotide incorporation by Dpo3 from the polymerase active site alone is 1000-10000 at 37°C. The functional exonuclease proofreading active site will increase fidelity by at least 100 Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 maximal activity is observed with 20 mM Tris between pH 7 and 8.5 Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function Dpo3 is a potential player in the proper maintenance of the archaeal genome Saccharolobus solfataricus