Protein Variants | Comment | Organism |
---|---|---|
D378A | site-directed mutagenesis, the mutant is inactive in presence of Mg2+ | Geobacillus kaustophilus |
D378E | site-directed mutagenesis, the mutant is inactive in presence of Mg2+, it shows 35% of maximal activity in presenceof Mn2+ | Geobacillus kaustophilus |
D380A | site-directed mutagenesis, the mutant is inactive in presence of Mg2+ | Geobacillus kaustophilus |
D380E | site-directed mutagenesis, the mutant is inactive in presence of Mg2+ or Mn2+ | Geobacillus kaustophilus |
D531A | site-directed mutagenesis, the mutant is inactive in presence of Mg2+ | Geobacillus kaustophilus |
D531E | site-directed mutagenesis, the mutant is inactive in presence of Mg2+, it shows 60% of maximal activity in presenceof Mn2+ | Geobacillus kaustophilus |
additional information | DNA binding, dNTP binding and catalytic activity of mutant enzyme in the presence of two metal ions, Mg2+ and Mn2+, overview | Geobacillus kaustophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Geobacillus kaustophilus | |
Mn2+ | activates | Geobacillus kaustophilus | |
additional information | DNA binding, dNTP binding and catalytic activity of mutant enzyme in the presence of two metal ions, Mg2+ and Mn2+, overview. Amino acid residues D378 and D531 are mainly responsible for the binding of metal chelated substrate dNTP | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
non-replicative DNA polymerase III | - |
Geobacillus kaustophilus |
pol E | - |
Geobacillus kaustophilus |
General Information | Comment | Organism |
---|---|---|
malfunction | abolished catalytic activity of mutant enzyme in the presence of two metal ions, Mg2+ and Mn2+, overview | Geobacillus kaustophilus |
additional information | three aspartic acid residues D378, D380 and D531 form the catalytic carboxylate triad in pol E. Amino acid residues D378 and D531 are mainly responsible for the binding of metal chelated substrate dNTP, while D380 is solely responsible for the chemical step of phosphodiester bond formation | Geobacillus kaustophilus |