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Literature summary for 2.7.7.7 extracted from
- Characterization of a Y-family DNA polymerase eta from the eukaryotic thermophile Alvinella pompejana (2010), J. Nucleic Acids, 2010, 1-13.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA polymerase eta phylogenetic analysis, expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus expression system | Alvinella pompejana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Alvinella pompejana | - |
diphosphate + DNAn+1 | - |
? |  |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | the enzyme retains activity in the presence of a higher concentration of organic solvents | Alvinella pompejana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alvinella pompejana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged enzyme from Sf9 cells by anion exchange, nickel affinity, and cation exchange chromatography | Alvinella pompejana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Alvinella pompejana | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase eta | - |
Alvinella pompejana |
| Poleta | - |
Alvinella pompejana |
| Y-family DNA polymerase eta | - |
Alvinella pompejana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Alvinella pompejana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme shows higher thermostability | Alvinella pompejana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Alvinella pompejana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the DNA polymerase Y-family, phylogenetic analysis | Alvinella pompejana |
| additional information | the enzyme contains domains for binding ubiquitin and proliferating cell nuclear antigen | Alvinella pompejana |
| physiological function | the DNA polymerase catalyzes efficient and accurate translesion synthesis past cis-syn cyclobutane pyrimidine dimers, as well as 7,8-dihydro-8-oxoguanine and isomers of thymine glycol induced by reactive oxygen species | Alvinella pompejana |
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Release 2023.1 (January 2023)
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