Organism | UniProt | Comment | Textmining |
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Saccharolobus solfataricus | - |
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- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | an abasic lesion causes Dpo4 to switch from a normal to a very mutagenic mode of replication. Incorporation upstream of the abasic lesion is replicated error-free. Once Dpo4 encounters the lesion, synthesis became sloppy, with bypass products containing a myriad of mutagenic events. Incorporation of dAMP (29%) and dCMP (53%) opposite the abasic lesion at 37°C correlates exceptionally well with our kinetic results and demonstrates two dominant bypass pathways via the A-rule and the lesion loop-out mechanism. The percentage of overall frameshift mutations increases from 71% (37°C) to 87% (75°C) | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
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deoxynucleoside triphosphate + DNAn | an abasic lesion causes the enzyme to switch from a normal to a very mutagenic mode of replication | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
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Synonyms | Comment | Organism |
---|---|---|
Dpo4 | - |
Saccharolobus solfataricus |
General Information | Comment | Organism |
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physiological function | DNA damage that eludes cellular repair pathways can arrest the replication machinery and stall the cell cycle. This damage can be bypassed by the Y-family DNA polymerases | Saccharolobus solfataricus |