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Literature summary for 2.7.7.7 extracted from
- Purification and characterization of a gamma-like DNA polymerase from Chenopodium album L (1993), Nucleic Acids Res., 21, 4893-4899.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-dideoxythymidine 5'-triphosphate | - |
Chenopodium album |  |
| K+ | optimum concentration 125 mM, inhibition at higher concentration | Chenopodium album | |
| additional information | no inhibition by aphidicolin; no inhibition by ara-CTP | Chenopodium album | |
| N-ethylmaleimide | inhibition at 1 mM | Chenopodium album | |
| phosphate | strong inhibitor | Chenopodium album | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Chenopodium album | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulates at 125 mM, inhibition at higher concentrations | Chenopodium album | |
| Mg2+ | optimal concentration: 10 mM | Chenopodium album | |
| Mg2+ | divalent cation required, Mg2+ or Mn2+ | Chenopodium album | |
| Mn2+ | divalent cation required, Mn2+ or Mg2+ | Chenopodium album | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | 90000 | glycerol density gradient sedimentation | Chenopodium album |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Chenopodium album | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chenopodium album | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gamma-like DNA polymerase | Chenopodium album |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.523 | - |
- |
Chenopodium album |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Chenopodium album | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity | Chenopodium album | diphosphate + DNAn+1 | - |
? | |
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