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Literature summary for 2.7.7.7 extracted from
- Characterization of DNA polymerase from the hyperthermophilic archaeon Thermococcus marinus and its application to PCR (2009), Extremophiles, 13, 657-667.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3) cells | Thermococcus marinus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | highly dependent on MgCl2 in the range of 0-20 mM with maximal activity at 14 mM MgCl2 and no detectable activity in the absence of MgCl2 | Thermococcus marinus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
about 90000 Da, mature DNA polymerase, SDS-PAGE | Thermococcus marinus |
| 152400 | - |
about 90000 Da, DNA polymerase precursor form, estimated from amino acid sequence | Thermococcus marinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus marinus | C7AIP4 | strain DSM 15227 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap heparin column chromatography, gel filtration | Thermococcus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Thermococcus marinus | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase | - |
Thermococcus marinus |
| Pol I | - |
Thermococcus marinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermococcus marinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 94 | 99 | the half life of the enzyme is about 2 h at 94°C and 45 min at 99°C | Thermococcus marinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in Tris-HCl buffer | Thermococcus marinus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the presence of 5'->3' exonuclease activity in DNA polymerase can replace mismatched base pairs with the correct nucleotide, DNA polymerase possesses 3'->5' exonuclease activity | Thermococcus marinus |
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