Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
D405A | mutant enzyme loses 99.8% of DNA polymerizing activity and 90% of 3'->5' exonucleolytic activity | Pyrococcus furiosus |
D405E | mutant enzyme loses 95.8% of DNA polymerizing activity and 90% of 3'->5' exonucleolytic activity | Pyrococcus furiosus |
DELTAH672-S775 | mutant enzyme loses 99% of DNA polymerizing activity and 97% of 3'->5' exonucleolytic activity | Pyrococcus furiosus |
DELTAL717-S775 | mutant enzyme loses 97% of DNA polymerizing activity and 97% of 3'->5' exonucleolytic activity | Pyrococcus furiosus |
DELTAL746-S775 | mutant protein has DNA polymerizing activity with 2.3fold higher specific activity than that of the wild-type but retains only 10% of the 3'->5' exonucleolytic activity of the wild-type | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | measurement of the incorporation of methyl-TTP into acid insoluble material. The single-stranded DNA substrate is more sensitive than the double stranded substrate. The polymerase and exonuclease domains in the family B DNA polymerases are functionally interdependent | Pyrococcus furiosus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase I | - |
Pyrococcus furiosus |
Pol BI | - |
Pyrococcus furiosus |