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Literature summary for 2.7.7.7 extracted from

  • Auer, T.; Landre, P.A.; Myers, T.W.
    Properties of the 5'->3' exonuclease/ribonuclease H activity of Thermus thermophilus DNA polymerase (1995), Biochemistry, 34, 4994-5002.
    View publication on PubMed
Search for more literature for 2.7.7.7

Application

Application Comment Organism
biotechnology thermostable polymerase used in PCR Thermus thermophilus
molecular biology thermostable polymerase used in PCR Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
dimethyl sulfoxide 67% inhibition of RNAse H activity at 10% Thermus thermophilus
glycerol 33% inhibition of RNAse H activity at 10% Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme prefers Mn2+ over Mg2+ for RNase H activity Thermus thermophilus
Mn2+ enzyme prefers Mn2+ over Mg2+ for RNase H activity, optimal concentration: 1.5-2.5 mM Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus thermophilus
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Thermus thermophilus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn enzyme also has RNAse H/exonuclease 5'--3' activity, enzyme prefers RNA/DNA substrate over DNA/DNA duplex Thermus thermophilus diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 75 optimal RNAse H activity at 70°C, sharp decline of activity above 75°C Thermus thermophilus