Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the gap-filling activity of pol IV is not enhanced by a 5'-phosphate on the downstream primer but is stimulated by a 5'-terminal synthetic abasic site | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
dATP | - |
Saccharomyces cerevisiae | |
0.024 | - |
dCTP | - |
Saccharomyces cerevisiae | |
0.139 | - |
rCTP | - |
Saccharomyces cerevisiae | |
0.189 | - |
rATP | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharomyces cerevisiae | role in non-homologous end joining of double strand breaks, perhaps including those with damaged ends, possible role for pol IV in base excision repair | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Saccharomyces cerevisiae | ? | - |
? | |
dCTP + DNAn | - |
Saccharomyces cerevisiae | ? | - |
? | |
deoxynucleoside triphosphate + DNAn | role in non-homologous end joining of double strand breaks, perhaps including those with damaged ends, possible role for pol IV in base excision repair | Saccharomyces cerevisiae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme has intrinsic 5'-2-deoxyribose-5-phosphate lyase activity. Pol IV has low processivity and can fill short gaps in DNA. The gap-filling activity of pol IV is not enhanced by a 5'-phosphate on the downstream primer but is stimulated by a 5'-terminal synthetic abasic site. Pol IV incorporates rNTPs into DNA with high efficiency relative to dNTPs. Pol IV is highly inaccurate, with an unusual error specificity indicating the ability to extend primer termini with limited homology | Saccharomyces cerevisiae | diphosphate + DNAn+1 | - |
? | |
rATP + DNAn | - |
Saccharomyces cerevisiae | ? | - |
? | |
rCTP + DNAn | - |
Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase IV | - |
Saccharomyces cerevisiae |
Pol IV | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
rATP | - |
Saccharomyces cerevisiae | |
0.09 | - |
rCTP | - |
Saccharomyces cerevisiae | |
0.14 | - |
dATP | - |
Saccharomyces cerevisiae | |
0.16 | - |
dCTP | - |
Saccharomyces cerevisiae |