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Literature summary for 2.7.7.7 extracted from

  • Takagi, M.; Nishioka, M.; Kakihara, H.; Kitabayashi, M.; Inoue, H.; Kawakami, B.; Oka, M.; Imanaka, T.
    Characterization of DNA polymerase from sp. strain KOD1 and its application to PCR (1997), Appl. Environ. Microbiol., 63, 4504-4510.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Application

Application Comment Organism
biotechnology thermostable polymerase used in PCR Thermococcus kodakarensis
molecular biology thermostable polymerase used in PCR Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermococcus kodakarensis
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis P77933
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Thermococcus kodakarensis diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 50% loss of activity after 12 h Thermococcus kodakarensis
100
-
 50% loss of activity after 3 h Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Thermococcus kodakarensis