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Literature summary for 2.7.7.7 extracted from

  • Torri, A.F.; Englund, P.T.
    Purification of a mitochondrial DNA polymerase from Crithidia fasciculata (1992), J. Biol. Chem., 267, 4786-4792.
    View publication on PubMed
Search for more literature for 2.7.7.7

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Crithidia fasciculata 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration, glycerol gradient centrifugation Crithidia fasciculata
43000
-
 1 * 43000, SDS-PAGE Crithidia fasciculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Crithidia fasciculata
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Crithidia fasciculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Crithidia fasciculata diphosphate + DNAn+1
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Crithidia fasciculata

pI Value

Organism Comment pI Value Maximum pI Value
Crithidia fasciculata 2 species with pI 7.6 and pI 8, twodimensional electrophoresis, 1st dimension: isoelectric focusing, pH gradient between pH 3.5 and 10, 2nd dimension: SDS-PAGE
-
 7.6
Crithidia fasciculata 2 species with pI 7.6 and pI 8, twodimensional electrophoresis, 1st dimension: isoelectric focusing, pH gradient between pH 3.5 and 10, 2nd dimension: SDS-PAGE
-
 8