Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Thermococcus marinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
mature DNA polymerase, SDS-PAGE | Thermococcus marinus |
152000 | - |
precursor form of DNA polymerase containing 62 kDa intein, SDS-PAGE | Thermococcus marinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermococcus marinus | the DNA polymerase gene of Thermococcus marinus contains an intein inserted at the pol-b site | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus marinus | C7AIP4 | strain DSM 15227 | - |
Purification (Comment) | Organism |
---|---|
HiTrap heparin column chromatography, gel filtration | Thermococcus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermococcus marinus | diphosphate + DNAn+1 | - |
? | |
additional information | the DNA polymerase gene of Thermococcus marinus contains an intein inserted at the pol-b site | Thermococcus marinus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase | - |
Thermococcus marinus |