Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharolobus solfataricus | two representative types of lesions: (i) 7,8-dihydro-8-oxoguanine, a small, highly prevalent lesion caused by oxidative damage; and (ii) bulky lesions derived from the environmental pre-carcinogen benzo[a]pyrene. The diol epoxide (+)-(7R,8S,9S,10R)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a]pyrene reacts largely, but not exclusively, with the exocyclic amino group of guanine to produce the major 10S (+) trans-anti-BP-N2-dG adduct, that is bypassed by Dpo4 | diphosphate + DNAn+1 | - |
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additional information | Saccharolobus solfataricus | replication cycle of Dpo4, and induced fit and translocation mechanisms, overview | ? | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W02 | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | two representative types of lesions: (i) 7,8-dihydro-8-oxoguanine, a small, highly prevalent lesion caused by oxidative damage; and (ii) bulky lesions derived from the environmental pre-carcinogen benzo[a]pyrene. The diol epoxide (+)-(7R,8S,9S,10R)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a]pyrene reacts largely, but not exclusively, with the exocyclic amino group of guanine to produce the major 10S (+) trans-anti-BP-N2-dG adduct, that is bypassed by Dpo4 | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | two representative types of lesions: (i) 7,8-dihydro-8-oxoguanine, a small, highly prevalent lesion caused by oxidative damage; and (ii) bulky lesions derived from the environmental pre-carcinogen benzo[a]pyrene, Dpo4 bypasses 8-oxoG accurately | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
additional information | replication cycle of Dpo4, and induced fit and translocation mechanisms, overview | Saccharolobus solfataricus | ? | - |
? | |
additional information | Dpo4 produces mismatch and frameshift mutations at benzo[a]pyrene-derived lesions, overview | Saccharolobus solfataricus | ? | - |
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Subunits | Comment | Organism |
---|---|---|
More | Dpo4 has a spacious, highly solvent-accessible active-site region, schematic and molecular surface representations of Dpo4 ternary structure, comparison to the high-fidelity DNA polymerase Bacillus fragment, overview | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase IV | - |
Saccharolobus solfataricus |
Dpo4 | - |
Saccharolobus solfataricus |
lesion-bypass DNA polymerase | - |
Saccharolobus solfataricus |
More | the enzyme belongs to the Y-family polymerases | Saccharolobus solfataricus |