Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Broyde, S.; Wang, L.; Rechkoblit, O.; Geacintov, N.E.; Patel, D.J.
    Lesion processing: high-fidelity versus lesion-bypass DNA polymerases (2008), Trends Biochem. Sci., 33, 209-219.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus two representative types of lesions: (i) 7,8-dihydro-8-oxoguanine, a small, highly prevalent lesion caused by oxidative damage; and (ii) bulky lesions derived from the environmental pre-carcinogen benzo[a]pyrene. The diol epoxide (+)-(7R,8S,9S,10R)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a]pyrene reacts largely, but not exclusively, with the exocyclic amino group of guanine to produce the major 10S (+) trans-anti-BP-N2-dG adduct, that is bypassed by Dpo4 diphosphate + DNAn+1
-
?
additional information Saccharolobus solfataricus replication cycle of Dpo4, and induced fit and translocation mechanisms, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97W02
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn two representative types of lesions: (i) 7,8-dihydro-8-oxoguanine, a small, highly prevalent lesion caused by oxidative damage; and (ii) bulky lesions derived from the environmental pre-carcinogen benzo[a]pyrene. The diol epoxide (+)-(7R,8S,9S,10R)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a]pyrene reacts largely, but not exclusively, with the exocyclic amino group of guanine to produce the major 10S (+) trans-anti-BP-N2-dG adduct, that is bypassed by Dpo4 Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn two representative types of lesions: (i) 7,8-dihydro-8-oxoguanine, a small, highly prevalent lesion caused by oxidative damage; and (ii) bulky lesions derived from the environmental pre-carcinogen benzo[a]pyrene, Dpo4 bypasses 8-oxoG accurately Saccharolobus solfataricus diphosphate + DNAn+1
-
?
additional information replication cycle of Dpo4, and induced fit and translocation mechanisms, overview Saccharolobus solfataricus ?
-
?
additional information Dpo4 produces mismatch and frameshift mutations at benzo[a]pyrene-derived lesions, overview Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
More Dpo4 has a spacious, highly solvent-accessible active-site region, schematic and molecular surface representations of Dpo4 ternary structure, comparison to the high-fidelity DNA polymerase Bacillus fragment, overview Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
DNA polymerase IV
-
Saccharolobus solfataricus
Dpo4
-
Saccharolobus solfataricus
lesion-bypass DNA polymerase
-
Saccharolobus solfataricus
More the enzyme belongs to the Y-family polymerases Saccharolobus solfataricus