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Information on EC 3.1.3.2 - acid phosphatase
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3.1.3.2 acid phosphataseIUBMB Comments
Wide specificity. Also catalyses transphosphorylations.
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Word Map
- 3.1.3.2
- lysosomal
- osteoclast
- resorption
-
cytochemical
- marrow
- collagen
- beta-glucuronidase
-
multinucleated
- rankl
-
osteoclastogenesis
- granule
- vacuole
- osteoporosis
-
osteocalcin
- monocyte
-
prostate-specific
- phagocytic
-
trabecular
-
cytochemistry
- phosphorus
-
seminal
- calcitonin
-
histochemistry
- metastases
- alveolar
-
hemolytic
- periodontal
- tibia
-
ovariectomized
-
resorb
-
osteoprotegerin
- parathyroid
- femur
-
bone-specific
-
n-acetyl-beta-d-glucosaminidase
-
telopeptide
-
cisternae
- dentin
-
histomorphometric
- sildenafil
- calvaria
- osteolysis
-
prostatectomy
-
hairy
-
erectile
- 5'-nucleotidase
- phytase
- glucose-6-phosphatase
-
transrectal
- molecular biology
- synthesis
- agriculture
- medicine
- biotechnology
- gaucher
- diagnostics
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
tartrate-resistant acid phosphatase, prostatic acid phosphatase, acid phosphatases, tracp, acpase, uteroferrin, tracp 5b, human prostatic acid phosphatase, tracp5b, pp2a phosphatase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acid monophosphatase
-
-
-
-
acid nucleoside diphosphate phosphatase
-
-
-
-
acid phosphatase
acid phosphatase A
acid phosphatase I
acid phosphatase II
acid phosphatase III
acid phosphatase MapA
Acid phosphatase PII
-
-
-
-
acid phosphatases
acid phosphohydrolase
-
-
-
-
acid phosphomoesterase
-
-
-
-
acid phosphomonoester hydrolase
-
-
-
-
ACP
ACP1
Acp5
AcPase
Adipocyte acid phosphatase, isozyme alpha
-
-
-
-
Adipocyte acid phosphatase, isozyme beta
-
-
-
-
AP
APase
APASE6
-
-
-
-
AphA
Apho1p
bNSAP
class C acid phosphatase
class-A bacterial non-specific acid phosphatases
glycerophosphatase
-
-
-
-
HPAP
-
-
-
-
IAP
intracellular acid phosphatase
LAP
-
-
-
-
Low molecular weight phosphotyrosine protein phosphatase
-
-
-
-
mAPA
Minor phosphate-irrepressible acid phosphatase
-
-
-
-
NapE
non-specific acid phosphatase
NSAP
-
-
-
-
orthophosphoric-monoester phosphohydrolase
P56
-
-
-
-
P60
-
-
-
-
PAP
pH 2.1 optimum acid phosphatase
pH 2.5 acid phosphatase
-
-
-
-
pH 6-optimum acid phosphatase
-
-
-
-
PhoN
phosphohydrolase of orthophosphoric acid monoesters (acid optimum)
phosphomonoesterase
-
-
-
-
prostatic acid phosphatase
purple acid phosphatase
rkbPAP
SAP1
SAP2
secreted acid phosphatase
Stationary-phase survival protein surE
-
-
-
-
Tartrate-resistant acid ATPase
-
-
-
-
tartrate-resistant acid phosphatase
TR-AP
-
-
-
-
TRAP
TrATPase
-
-
-
-
uteroferrin
additional information
acid phosphatase MapA
autotransporter with acid phosphatase activity
acid phosphatase MapA
A9XED2
autotransporter with acid phosphatase activity
-
acid phosphatase MapA
autotransporter with acid phosphatase activity
-
acid phosphatase MapA
autotransporter with acid phosphatase activity
-
acid phosphatase MapA
A9XDF5
autotransporter with acid phosphatase activity
-
acid phosphatase MapA
autotransporter with acid phosphatase activity
-
ACP1
-
-
-
-
AcPase
-
-
-
-
APase
-
-
-
-
class-A bacterial non-specific acid phosphatases
-
class-A bacterial non-specific acid phosphatases
-
-
PAP
-
-
-
-
purple acid phosphatase
-
-
uteroferrin
-
-
-
-
additional information
-
the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases
additional information
the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases
additional information
VSPalpha belongs to the haloacid dehalogenase superfamily
additional information
-
the enzyme belongs to the family of binuclear metalloenzymes
additional information
Q764C1
the colorless acid phosphatase is distinct from the purple acid phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a phosphate monoester + H2O = an alcohol + phosphate
formation of a covalent phosphoryl-enzyme intermediate, kinetic pattern of a pseudo uni-bi hydrolytic two-step reaction mechanism
-
a phosphate monoester + H2O = an alcohol + phosphate
regioselective and stereospecific phosphorylation of diverse substrates by the acid phosphatase from Shigella flexneri and Salmonella enterica
-
a phosphate monoester + H2O = an alcohol + phosphate
regioselective and stereospecific phosphorylation of diverse substrates by the acid phosphatase from Shigella flexneri and Salmonella enterica
-
a phosphate monoester + H2O = an alcohol + phosphate
modelling of substrate binding in the active site
a phosphate monoester + H2O = an alcohol + phosphate
Asn91 and His92 are active site residues which interact with the loop residue Asp146, this interaction and substrate positioning by His92 is important for catalysis, Asn91 ia a ligand to the divalent metal of the mixed-valent di-iron center
-
a phosphate monoester + H2O = an alcohol + phosphate
the enzyme is a Zn-metallophosphatase containing an essential zinc ion in the active site
-
a phosphate monoester + H2O = an alcohol + phosphate
detailed structure-based catalytic mechanism
a phosphate monoester + H2O = an alcohol + phosphate
active site structure analysis, substrate binding, H295 and E365 are involved in substrate orientation and stabilization of the transition state, oxygen binding and bridging at the metal ion center, overview, reaction mechanism
a phosphate monoester + H2O = an alcohol + phosphate
the dephosphorylation of phosphotyrosine by PAP is a two-step process: the first step involves the transfer of a phosphate group from the substrate to the histidine (His12). A number of quantum models are constructed containing various residues that are thought to play a role in the mechanism. In all these models, the transition state displays an associative character. The transition state is stabilized by three active site arginines (Arg11, Arg15, and Arg79), two of which belong to the conserved motif (RHGXRXP). His12 can act as a nucleophile. The enzyme is further characterized by a His257-Asp258 motif. Asp258 acts as a proton donor which becomes protonated when the substrate enters the binding pocket. The transfer of a proton from Asp258 to the leaving group is possibly mediated by a water molecule in the active site. The importance of His257 in lowering the energy barrier for the nucleophilic attack is shown
-
a phosphate monoester + H2O = an alcohol + phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
phosphomonoester
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
phosphate-monoester phosphohydrolase (acid optimum)
Wide specificity. Also catalyses transphosphorylations.
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CAS REGISTRY NUMBER
COMMENTARY
9001-77-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-oleoyl lysophosphatidic acid + H2O
1-oleoyl glycerol + phosphate
-
further substrates: lauroyl-, myristoyl-, palmitoyl- and stearoyl lysophosphatidic acid
-
-
?
2,2-dichlorovinyl dimethylphosphate + H2O
?
-
i.e. dichlorvos, an organophosphate pesticide, complete degradation
-
-
?
2,3-bisphosphoglycerate + H2O
?
-
85% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerate + phosphate
-
127% of the activity with myo-inositol hexakisphosphate
-
-
?
2-phosphoenolpyruvate + H2O
enolpyruvate + phosphate
-
75.6% activity compared to 4-nitrophenyl phosphate
-
-
?
2-phosphoglycerate + H2O
glycerol + phosphate
-
32% activity compared to p-nitrophenyl phosphate
-
-
?
3-phosphoglycerate + H2O
glycerol + phosphate
-
34% activity compared to p-nitrophenyl phosphate
-
-
?
4-nitrophenyl phosphorylcholine + H2O
4-nitrophenyl choline + phosphate
-
at pH 7.4,synthetic substrate
-
-
?
5'-ADP + H2O
5'-AMP + phosphate
-
24% of the activity with 4-nitrophenyl phosphate
-
-
?
5'-IMP + H2O
?
-
isoenzyme AP1 and AP-2, no activity with isoenzyme AP-3 and AP-4
-
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O
5-bromo-4-chloro-3-hydroxyindole + phosphate
-
-
-
-
?
5-hydroxyethyl-4-methylthiazole phosphate + H2O
5-hydroxyethyl-4-methylthiazole + phosphate
-
-
-
?
6,8 difluoro-4-methylumbelliferyl phosphate + H2O
6,8 difluoro-4-methylumbelliferol + phosphate
-
-
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
6,8-difluoro-4-methylumbelliferone + phosphate
6-phospho-D-gluconate + H2O
D-gluconate + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
6-phosphogluconate + H2O
gluconate + phosphate
-
17% of the activity with p-nitrophenyl phosphate
-
-
?
a pyridine nucleoside 5'-triphosphate + H2O
a pyridine nucleoside 5'-diphosphate + phosphate
-
-
-
-
?
Ac-RK(pS)AGKPKN + H2O
?
synthetic phosphorylated oligopeptide
-
-
?
adenosine monophosphate + H2O
adenosine + phosphate
-
relative hydrolytic rate: 50%
-
-
?
adenosine triphosphate + H2O
ATP + phosphate
-
relative hydrolytic rate: 25%
-
-
?
alpha-D-glucose 1-phosphate + H2O
alpha-D-glucose + phosphate
-
9% of the activity with 4-nitrophenyl phosphate
-
?
alpha-D-glucose 6-phosphate + H2O
alpha-D-glucose + phosphate
-
58% of the activity with 4-nitrophenyl phosphate
-
?
alpha-naphthylphosphate + H2O
alpha-naphthol + phosphate
-
32% activity compared to p-nitrophenyl phosphate
-
-
?
ATP + H2O
? + phosphate
-
256% of the activity with myo-inositol hexakisphosphate
-
-
?
beta-naphthylphosphate + H2O
beta-naphthol + phosphate
-
59% activity compared to p-nitrophenyl phosphate
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
choline O-phosphate + H2O
choline + phosphate
-
9% of the activity with p-nitrophenyl phosphate
-
-
?
coenzyme A + H2O
?
-
43% of the activity with p-nitrophenyl phosphate
-
-
?
cytidine monophosphate + H2O
cytidine + phosphate
-
relative hydrolytic rate: 47%
-
-
?
D-glucose-1-phosphate + H2O
D-glucose + phosphate
-
relative hydrolytic rate: 13%
-
-
?
D-glucose-6-phosphate + H2O
D-glucose + phosphate
-
relative hydrolytic rate: 14%
-
-
?
D-mannose 6-phosphate + H2O
D-mannose + phosphate
-
85% of the activity with 4-methylumbelliferyl phosphate
-
-
?
DADE(pY)LIPQQG + H2O
DADEYLIPQQG + phosphate
-
tyrosine phosphopeptide
-
-
?
dAMP + H2O
2'-deoxyadenosine + phosphate
16% activity compared to phosphoenolpyruvate
-
-
?
dATP + H2O
? + phosphate
31% of the activity with 4-nitrophenylphosphate
-
-
?
dCTP + H2O
? + phosphate
35% of the activity with 4-nitrophenylphosphate
-
-
?
deoxythymidine 3'-(p-nitrophenyl)phosphate + H2O
?
-
5% of the activity with p-nitrophenyl phosphate
-
-
?
deoxythymidine 5'-(p-nitrophenyl) phosphate + H2O
?
-
10% of the activity with p-nitrophenyl phosphate
-
-
?
dGTP + H2O
? + phosphate
52% of the activity with 4-nitrophenylphosphate
-
-
?
dihydroxyacetone phosphate + H2O
dihydroxyacetone + phosphate
-
isoenzyme AP-1 and AP-2, weak activity with isoenzyme AP-4, no activity with isoenzyme AP-3
-
-
?
DL-myo-inositol-1-monophosphate + H2O
DL-myo-inositol + phosphate
-
-
-
?
dodecasodium phytate + H2O
? + phosphate
-
245% activity compared to 4-nitrophenyl phosphate
-
-
?
dTTP + H2O
? + phosphate
37% of the activity with 4-nitrophenylphosphate
-
-
?
glyceraldehyde-3-phosphoric acid diethylacetal + H2O
?
Dioscorea cayenensis-rotundata
-
-
-
-
?
guanosine monophosphate + H2O
guanosine + phosphate
-
relative hydrolytic rate: 32%
-
-
?
hirudin 53-65 C-terminal fragment + H2O
?
-
containing phosphotyrosine63
-
?
inositol 1,4,5-triphosphate + H2O
?
-
15% of the activity with 4-methylumbelliferyl phosphate
-
-
?
inositol 4-phosphate + H2O
inositol + phosphate
-
28% of the activity with 4-methylumbelliferyl phosphate
-
-
?
inositol cyclic phosphate + H2O
?
-
3% of the activity with 4-methylumbelliferyl phosphate
-
-
?
ITP + H2O
IDP + phosphate
-
85% of the activity with p-nitrophenyl phosphate
-
-
?
myo-inositol 2-monophosphate + H2O
myo-inositol + phosphate
-
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
N-acetylglucosamine 1-phosphate + H2O
N-acetylglucosamine + phosphate
-
-
-
-
?
naphthyl phosphate + H2O
?
-
48% of the activity with p-nitrophenyl phosphate
-
-
?
O,O,O,O-tetraethyldithiodiphosphate + H2O
?
-
i.e. sulfatep, an organophosphate pesticide, 50% degradation
-
-
?
O,O-dimethyl O-4-nitrophenyl phosphate + H2O
?
-
i.e. paraoxon, an organophosphate pesticide
-
-
?
O-2-chloro-4-nitrophenyl O,O-dimethylphosphorothionate + H2O
?
-
i.e. dicapthon, an organophosphate pesticide, complete degradation
-
-
?
o-nitrophenyl phosphate + H2O
o-nitrophenol + phosphate
-
21% of the activity with p-nitrophenylphosphate
-
-
?
O-phospho-DL-serine + H2O
?
-
16% of the activity with 4-nitrophenyl phosphate
-
?
O-phospho-DL-threonine + H2O
?
-
5% of the activity with 4-nitrophenyl phosphate
-
?
O-phospho-DL-tyrosine + H2O
?
-
4% of the activity with 4-nitrophenyl phosphate
-
?
O-phospho-L-serine + H2O
phosphate + L-serine
-
45% of the activity with O-phospho-L-serine
-
?
O-phospho-L-threonine + H2O
phosphate + L-threonine
-
10% of the activity with O-phospho-L-serine
-
?
O-phospho-L-tyrosine + H2O
L-tyrosine + phosphate
28% of the activity with 4-nitrophenylphosphate
-
-
?
p-nitrophenyl phenylphosphonate + H2O
?
-
160% of the activity with p-nitrophenyl phosphate
-
-
?
p-nitrophenyl phosphate + 2'-deoxyadenosine
?
-
phosphotransferase activity
-
-
?
p-nitrophenyl phosphate + 2'-deoxycytidine
?
-
phosphotransferase activity
-
-
?
p-nitrophenyl phosphate + 2'-deoxyuridine
?
-
phosphotransferase activity
-
-
?
p-nitrophenyl phosphate + adenosine
?
-
phosphotransferase activity
-
-
?
p-nitrophenyl phosphate + uridine
?
-
phosphotransferase activity
-
-
?
p-nitrophenyl phosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
phospho-myelin basic protein + H2O
myelin basic protein + phosphate
-
isozyme 2
-
-
?
phosphorylated-ErbB-2 + H2O
?
-
cPAcP interacts with and dephosphorylates ErbB-2 primarily at Tyr1221/2 and hence blocks downstream signaling, leading to reduced cell growth
-
-
?
phosphotyrosyl histone + H2O
histone + phosphate
-
4.2% of the activity with p-nitrophenyl phosphate
-
-
?
polyphosphate + H2O
?
-
72% of the activity with 4-nitrophenyl phosphate
-
?
pyridoxamine 5'-phosphate + H2O
pyridoxamine + phosphate
-
-
about 50% of the activity with pyridoxal 5'-phosphate
-
?
pyridoxine 5'-phosphate + H2O
pyridoxine + phosphate
-
-
about 50% of the activity with pyridoxal 5'-phosphate
-
?
rabbit 32P-phosphorylase a + H2O
?
-
protein containing phosphoserine, poor substrate
-
?
S-1,2-bis(ethoxycarbonyl)ethyl O,O-dimethylphosphorodithionate + H2O
?
-
i.e. malathion, an organophosphate pesticide, 95% degradation
-
-
?
S-2-ethylthioethyl O,O-dimethylphosphothionate + H2O
?
-
i.e. demeton-S-methyl, an organophosphate pesticide, 36% degradation
-
-
?
thiamine diphosphate + H2O
?
-
76% of the activity with p-nitrophenyl phosphate
-
-
?
trimetaphosphate + H2O
?
-
7% of activity with p-nitrophenyl phosphate
-
-
?
uridine monophosphate + H2O
uridine + phosphate
-
relative hydrolytic rate: 41%
-
-
?
1-glycerate phosphate + H2O
glycerate + phosphate
-
19% of the activity with p-nitrophenyl phosphate
-
-
?
1-glycerate phosphate + H2O
glycerate + phosphate
-
isoenzyme AP-2, no activity with isoenzyme AP-1, AP-3 and AP-4
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
30% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
86% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
163% activity compared to 4-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
163% activity compared to 4-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
Dioscorea cayenensis-rotundata
-
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
60% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
-
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
most rapidly hydrolyzed
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
71% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
80% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
5% of the activity with 4-nitrophenyl phosphate
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
isoenzyme S4 and S1, no activity with isoenzyme S3, S2, and Szn
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
37% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
12% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
reaction with SAP1 and SAP2
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
0.7% of the activity with p-nitrophenyl phosphate
-
-
?
1-naphthyl phosphate + H2O
naphthol + phosphate
-
relative activity: 43% compared to phosphoenolpyruvate as substrate
-
-
?
1-naphthyl phosphate + H2O
naphthol + phosphate
-
relative activity: 53% compared to phosphoenolpyruvate as substrate
-
-
?
2'-AMP + H2O
adenosine + phosphate
-
6% of the activity with p-nitrophenyl phosphate
-
-
?
2'-AMP + H2O
adenosine + phosphate
-
7% of the activity with p-nitrophenyl phosphate
-
-
?
2,3-diphosphoglycerate + H2O
?
-
hydrolysed by basic enzyme form, no activity with acidic enzyme form
-
-
?
2,3-diphosphoglycerate + H2O
?
-
isoenzyme AP-2, no activity with isoenzyme AP-1, AP-3 and AP-4
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
40% of the activity with p-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
17% of the activity with p-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
72% of the activity with p-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
Dioscorea cayenensis-rotundata
-
enzyme B and C, no activity with enzyme A
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
-
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
69% of the activity with p-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
37% of the activity with p-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
8% of the activity with p-nitrophenyl phosphate
-
-
?
2-glycerophosphate + H2O
glycerol + phosphate
-
isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
relative activity: 116% compared to phosphoenolpyruvate as substrate
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
relative activity: 118% compared to phosphoenolpyruvate as substrate
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
85% of the activity with p-nitrophenyl phosphate
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
Dioscorea cayenensis-rotundata
-
-
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
105% of the activity with p-nitrophenyl phosphate
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
26% of the activity with 4-nitrophenyl phosphate
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
26% of the activity with 4-nitrophenyl phosphate
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
reaction with SAP1 and SAP2
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
35% of the activity with p-nitrophenyl phosphate
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
59% of the activity with 4-nitrophenyl phosphate
-
-
?
2-phosphoglycerate + H2O
glycerate + phosphate
-
80.9% activity compared to 4-nitrophenyl phosphate
-
-
?
2-phosphoglycerate + H2O
glycerate + phosphate
-
80.9% activity compared to 4-nitrophenyl phosphate
-
-
?
3'-AMP + H2O
adenosine + phosphate
-
68% of the activity with p-nitrophenyl phosphate
-
-
?
3'-AMP + H2O
adenosine + phosphate
-
60% of the activity with p-nitrophenyl phosphate
-
-
?
3'-AMP + H2O
adenosine + phosphate
-
53% of the activity with p-nitrophenyl phosphate
-
-
?
3'-AMP + H2O
adenosine + phosphate
-
isoenzyme S4, weak activity with isoenzyme S1, no activity with isoenzyme S2, S3 and Szn
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
69% of the activity with 4-nitrophenylphosphate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
-
relative activity: 38% compared to phosphoenolpyruvate as substrate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
-
relative activity: 67% compared to phosphoenolpyruvate as substrate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferol + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
82% of the activity with p-nitrophenyl phosphate
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
relative activity: 101% compared to phosphoenolpyruvate as substrate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
relative activity: 83% compared to phosphoenolpyruvate as substrate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
higher activity with extracellular enzyme
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
254% of the activity with myo-inositol hexakisphosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
100% activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
100% activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
isozymes 1 and 2
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
relative hydrolytic rate: 100%
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
A9XDF5
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
about 158% of the activity with pyridoxal 5'-phosphate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
high activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
best substrate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
reaction with SAP1 and SAP2
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
5'-ADP + H2O
?
-
3% of the activity with 4-nitrophenyl phosphate
-
?
5'-ADP + H2O
?
-
3% of the activity with 4-nitrophenyl phosphate
-
?
5'-ADP + H2O
AMP + phosphate
-
14% of the activity with p-nitrophenyl phosphate
-
-
?
5'-ADP + H2O
AMP + phosphate
-
15% of the activity with p-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
72% of the activity with p-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
105% of the activity with p-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
83% of the activity with alpha-naphthyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
3% of the activity with p-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
13% of the activity with 4-nitrophenyl phosphate
-
?
5'-AMP + H2O
adenosine + phosphate
-
13% of the activity with 4-nitrophenyl phosphate
-
?
5'-AMP + H2O
adenosine + phosphate
-
15% of the activity with p-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
38% of the activity with 4-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4
-
-
?
5'-ATP + H2O
?
-
18% of the activity with 4-nitrophenyl phosphate
-
?
5'-ATP + H2O
?
-
18% of the activity with 4-nitrophenyl phosphate
-
?
5'-CMP + H2O
cytidine + phosphate
-
46% of the activity with p-nitrophenyl phosphate
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
97% of the activity with p-nitrophenyl phosphate
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
52% of the activity with p-nitrophenyl phosphate
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
33% of the activity with p-nitrophenyl phosphate
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
5% of the activity with p-nitrophenyl phosphate
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4
-
-
?
5'-UMP + H2O
uridine + phosphate
-
10% of the activity with p-nitrophenyl phosphate
-
-
?
5'-UMP + H2O
uridine + phosphate
-
5% of the activity with p-nitrophenyl phosphate
-
-
?
5'-UMP + H2O
uridine + phosphate
-
isoenzyme AP-1 and AP-2, very low activity with isoenzyme AP-3 and AP-4
-
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
6,8-difluoro-4-methylumbelliferone + phosphate
-
-
-
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
6,8-difluoro-4-methylumbelliferone + phosphate
-
-
-
?
6-phospho-D-gluconate + H2O
gluconate + phosphate
-
relative activity: 67% compared to phosphoenolpyruvate as substrate
-
-
?
6-phospho-D-gluconate + H2O
gluconate + phosphate
-
relative activity: 69% compared to phosphoenolpyruvate as substrate
-
-
?
adenosine-2',3'-cyclomonophosphate + H2O
?
-
63% activity compared to p-nitrophenyl phosphate
-
-
?
adenosine-2',3'-cyclomonophosphate + H2O
?
-
9% activity compared to p-nitrophenyl phosphate
-
-
?
ADP + H2O
?
-
58% of the activity with 4-nitrophenyl phosphate
-
?
ADP + H2O
? + phosphate
50% of the activity with 4-nitrophenylphosphate
-
-
?
ADP + H2O
? + phosphate
-
177% of the activity with myo-inositol hexakisphosphate
-
-
?
ADP + H2O
AMP + phosphate
-
relative activity: 68% compared to phosphoenolpyruvate as substrate
-
-
?
ADP + H2O
AMP + phosphate
-
relative activity: 77% compared to phosphoenolpyruvate as substrate
-
-
?
ADP + H2O
AMP + phosphate
-
100.2% activity compared to p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
103.4% activity compared to p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
81.5% activity compared to p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
36% of the activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
at 12.9% of the activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
52% of the activity with alpha-naphthyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
100% hydrolysis compared to hydrolysis of 4-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
92% of the activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
20% of the activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
isoenzyme S4 and S1, no activity with isoenzyme S3
-
-
?
ADP + H2O
AMP + phosphate
-
54% activity compared to p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
68% of activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
78% of activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
102% of the activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
19% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 33% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed
-
-
?
ADP + H2O
AMP + phosphate
-
10.4% of the activity with p-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
isoenzyme AP-1 and AP-3, very low activity with isoenzyme AP-4, no activity with isoenzyme AP-2
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
38% of the activity with p-nitrophenyl phosphate
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
15% of the activity with p-nitrophenylphosphate
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
Dioscorea cayenensis-rotundata
-
enzyme B and C, no activity with enzyme A
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
51% of the activity with alpha-naphthyl phosphate
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
48% of the activity with p-nitrophenyl phosphate
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
relative hydrolytic rate: 13%
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
relative hydrolytic rate: 15%
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
relative hydrolytic rate: 19%
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
-
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
isoenzyme AP-1 and AP-2, no activity with isoenzyme AP-3 and AP-4
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
-
high activity
-
-
?
alpha-naphthyl phosphate + H2O
naphthol + phosphate
-
relative hydrolytic rate: 3%
-
-
?
alpha-naphthyl phosphate + H2O
naphthol + phosphate
-
relative hydrolytic rate: 8%
-
-
?
alpha-naphthyl phosphate + H2O
naphthol + phosphate
-
relative hydrolytic rate: 88%
-
-
?
AMP + H2O
adenosine + phosphate
9% activity compared to phosphoenolpyruvate
-
-
?
AMP + H2O
adenosine + phosphate
-
25.2% activity compared to p-nitrophenyl phosphate
-
-
?
AMP + H2O
adenosine + phosphate
-
25.5% activity compared to p-nitrophenyl phosphate
-
-
?
AMP + H2O
adenosine + phosphate
-
51.5% activity compared to p-nitrophenyl phosphate
-
-
?
AMP + H2O
adenosine + phosphate
-
14% of the activity with p-nitrophenyl phosphate
-
-
?
AMP + H2O
adenosine + phosphate
-
173% of the activity with myo-inositol hexakisphosphate