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Literature summary for 3.1.3.2 extracted from

  • Wang, L.; Li, Z.; Qian, W.; Guo, W.; Gao, X.; Huang, L.; Wang, H.; Zhu, H.; Wu, J.W.; Wang, D.; Liu, D.
    Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation (2011), Plant Physiol., 157, 1283-99.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-fusion protein Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A385V mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
D167N mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
E340K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
E363K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
G169E mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
G195D mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
G27E mutation not in metallophosphatase domain results in partial loss of enzymatic activity Arabidopsis thaliana
G420R mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
G55D mutation not metallophosphatase domain results in partial loss of enzymatic activity Arabidopsis thaliana
G67R mutation not metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
P250L mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
R274K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
R415K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana
R455K mutation in metallophosphatase domain results in complete loss of enzymatic activity Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe Fe and Zn show a molar ratio of 1:0.75. AtPAP10 contains a bimetal nucleus composed of Fe and Zn Arabidopsis thaliana
Zn Fe and Zn show a molar ratio of 1:0.75. AtPAP10 contains a bimetal nucleus composed of Fe and Zn Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54200
-
 predicted from cDNA Arabidopsis thaliana
70000
-
 SDS-PAGE, GST fusion protein Arabidopsis thaliana
250000
-
 oligomer structure, using in-gel assays (electrophoresis under non-reducing conditions) Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography and gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
root AtPAP10 is predominantly associated with the root surface after secretion rather than being released into the rhizosphere Arabidopsis thaliana
-
silique
-
 Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
5-bromo-4-chloro-3-indolyl phosphate + H2O
-
 Arabidopsis thaliana 5-bromo-4-chloro-3-hydroxyindole + phosphate
-
 ?
ADP + H2O
-
 Arabidopsis thaliana AMP + phosphate
-
 ?
ATP + H2O
-
 Arabidopsis thaliana ADP + phosphate
-
 ?
dATP + H2O
-
 Arabidopsis thaliana dADP + phosphate
-
 ?
diphosphate + H2O
-
 Arabidopsis thaliana phosphate
-
 ?
L-phosphoserine + H2O
-
 Arabidopsis thaliana serine + phosphate
-
 ?
polyphosphate + H2O
-
 Arabidopsis thaliana phosphate
-
 ?

Subunits

Subunits Comment Organism
oligomer electrophoresis under non-reducing conditions Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtPAP10
-
 Arabidopsis thaliana
purple acid phosphatase 10
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 highest activity Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 active between Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 optimal activity between pH 5-6 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression of AtPAP10 is specifically induced by phosphate limitation at both transcriptional and posttranscriptional levels up

General Information

General Information Comment Organism
malfunction functional analyses of multiple atpap10 mutant alleles and overexpressing lines show that AtPAP10 plays an important role in plant tolerance to phosphate limitation Arabidopsis thaliana
malfunction growth of Atpap10 mutants is reduced under phosphate limitation Arabidopsis thaliana