Literature summary for 3.1.3.2 extracted from

Elliott, T.W.; Mitic, N.; Gahan, L.R.; Guddat, L.W.; Schenk, G.
Inhibition studies of purple acid phosphatases: implications for the catalytic mechanism (2006), J. Braz. Chem. Soc., 17, 1558-1565.

No PubMed abstract available

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Inhibitors

Inhibitors	Comment	Organism	Structure
fluoride	competitive inhibitor independent of pH	Phaseolus vulgaris
fluoride	competitive inhibitor independent of pH	Sus scrofa
vanadate	-	Phaseolus vulgaris
vanadate	non-competitive inhibitor at low pH and non-competitive inhibitor at higher pH	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.6	-	p-nitrophenyl phosphate	at pH 4.9	Phaseolus vulgaris
3.7	-	p-nitrophenyl phosphate	at pH 4.9	Sus scrofa
5.9	-	p-nitrophenyl phosphate	at pH 6.2	Phaseolus vulgaris
7	-	p-nitrophenyl phosphate	at pH 6.2	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Phaseolus vulgaris	-	variety red kidney bean	-
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
CM-cellulose resin chromatography and Sephadex G-75 gel filtration	Sus scrofa
CM-cellulose resin chromatography and Sephadex S-300 gel filtration	Phaseolus vulgaris

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Phaseolus vulgaris	-
uterine fluid	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl phosphate + H2O	-	Phaseolus vulgaris	p-nitrophenol + phosphate	-	?
p-nitrophenyl phosphate + H2O	-	Sus scrofa	p-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PAP	-	Phaseolus vulgaris
PAP	-	Sus scrofa
purple acid phosphatase	-	Phaseolus vulgaris
purple acid phosphatase	-	Sus scrofa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.9	-	-	Sus scrofa
6.2	-	-	Phaseolus vulgaris

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0295	-	vanadate	competitive inhibition at pH 6.2	Phaseolus vulgaris
0.04	-	vanadate	at pH 5.5	Sus scrofa
1.1	-	fluoride	competitive inhibition at pH 6.2	Phaseolus vulgaris
5.99	-	fluoride	competitive inhibition at pH 6.2	Sus scrofa

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Release 2023.1 (January 2023)