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Literature summary for 3.1.3.2 extracted from
- Purple acid phosphatase in the walls of tobacco cells (2008), Phytochemistry, 69, 2546-2551.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| brefeldin A | blocks secretion during wall regeneration | Nicotiana tabacum |  |
| molybdate | - |
Nicotiana tabacum | |
| NaF | - |
Nicotiana tabacum | |
| vanadate | - |
Nicotiana tabacum | |
| ZnCl2 | - |
Nicotiana tabacum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
DADE(pY)LIPQQG | pH 5.8, 30°C | Nicotiana tabacum | |
| 0.03 | - |
END(pY)INASL | pH 5.8, 30°C | Nicotiana tabacum | |
| 0.22 | - |
RRA(pT)VA | pH 5.8, 30°C | Nicotiana tabacum | |
| 0.33 | - |
p-nitrophenyl phosphate | pH 5.8, 30°C | Nicotiana tabacum | |
| 0.4 | - |
ATP | pH 5.8, 30°C | Nicotiana tabacum | |
| 0.4 | - |
phosphoserine | pH 5.8, 30°C | Nicotiana tabacum | |
| 0.41 | - |
phosphotyrosine | pH 5.8, 30°C | Nicotiana tabacum | |
| 1.45 | - |
IDP | pH 5.8, 30°C | Nicotiana tabacum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | composed as a homotetramer | Nicotiana tabacum | 5618 | - |
| cytoplasm | composed as a homodimer | Nicotiana tabacum | 5737 | - |
| additional information | double-immunofluorescence labeling results show that the enzyme might be translocated through the Golgi apparatus to the walls at the interphase and to the cell plate during cytokinesis | Nicotiana tabacum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | enzyme contains | Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000, SDS-PAGE in presence of dithiothreitol, in cytoplasm | Nicotiana tabacum |
| 60000 | - |
4 * 60000, SDS-PAGE in presence of dithiothreitol, in the walls | Nicotiana tabacum |
| 220000 | - |
tetramer, SDS-PAGE under non-reducing conditions | Nicotiana tabacum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acid phosphatase is eluted from a hydroxyapatite column. First and second butyl-Toyopearl columns are subjected to gel filtration on Superdex 200. The enzyme is purified 213fold | Nicotiana tabacum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 494 | - |
after final purification step | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Nicotiana tabacum | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | - |
Nicotiana tabacum | AMP + phosphate | - |
? | |
| ATP + H2O | - |
Nicotiana tabacum | ? | - |
? | |
| DADE(pY)LIPQQG + H2O | tyrosine phosphopeptide | Nicotiana tabacum | DADEYLIPQQG + phosphate | - |
? | |
| END(pY)INASL + H2O | tyrosine phosphopeptide | Nicotiana tabacum | ENDYINASL + phosphate | - |
? | |
| IDP + H2O | - |
Nicotiana tabacum | IMP + phosphate | - |
? | |
| phosphoserine + H2O | - |
Nicotiana tabacum | serine + phosphate | - |
? | |
| phosphotyrosine + H2O | - |
Nicotiana tabacum | tyrosine + phosphate | - |
? | |
| RRA(pT)VA + H2O | serine/threonine phosphopeptide | Nicotiana tabacum | RRATVA + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 60000, SDS-PAGE in presence of dithiothreitol, in cytoplasm | Nicotiana tabacum |
| homotetramer | 4 * 60000, SDS-PAGE in presence of dithiothreitol, in the walls | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| purple acid phosphatase | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana tabacum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
RRA(pT)VA | pH 5.8, 30°C | Nicotiana tabacum | |
| 26.7 | - |
DADE(pY)LIPQQG | pH 5.8, 30°C | Nicotiana tabacum | |
| 26.8 | - |
phosphoserine | pH 5.8, 30°C | Nicotiana tabacum | |
| 32.8 | - |
END(pY)INASL | pH 5.8, 30°C | Nicotiana tabacum | |
| 60.4 | - |
IDP | pH 5.8, 30°C | Nicotiana tabacum | |
| 62.4 | - |
phosphotyrosine | pH 5.8, 30°C | Nicotiana tabacum | |
| 125 | - |
p-nitrophenyl phosphate | pH 5.8, 30°C | Nicotiana tabacum | |
| 133.2 | - |
ATP | pH 5.8, 30°C | Nicotiana tabacum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
assay at | Nicotiana tabacum |
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