Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21 AI | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
D100A | 1% wild-type activity | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Clostridium perfringens | |
Ce3+ | - |
Clostridium perfringens | |
guanosine | - |
Clostridium perfringens | |
Hg2+ | - |
Clostridium perfringens | |
molybdate | - |
Clostridium perfringens | |
NaF | - |
Clostridium perfringens | |
Pb2+ | - |
Clostridium perfringens | |
ribose | - |
Clostridium perfringens | |
Sm2+ | - |
Clostridium perfringens | |
Tartrate | - |
Clostridium perfringens | |
vanadate | - |
Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.221 | - |
5'-UMP | - |
Clostridium perfringens | |
0.222 | - |
5'-TMP | - |
Clostridium perfringens | |
0.269 | - |
5'-AMP | - |
Clostridium perfringens | |
0.283 | - |
5'-GMP | - |
Clostridium perfringens | |
0.658 | - |
p-nitrophenyl phosphate | - |
Clostridium perfringens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Clostridium perfringens | |
Cr2+ | - |
Clostridium perfringens | |
Cu2+ | - |
Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, analytical ultracentrifugation, gel filtration, SDS-PAGE | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | B1Q002 | ATCC 10543 | - |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography | Clostridium perfringens |
Renatured (Comment) | Organism |
---|---|
- |
Clostridium perfringens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46.5 | - |
substrate 5'-AMP | Clostridium perfringens |
47.4 | - |
substrate 5'-TMP | Clostridium perfringens |
52.1 | - |
substrate 5'-GMP | Clostridium perfringens |
71.4 | - |
substrate 5'-UMP | Clostridium perfringens |
98.3 | - |
substrate p-nitrophenyl phosphate | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-TMP + H2O | - |
Clostridium perfringens | thymidine + phosphate | - |
? | |
4-methylumbelliferyl phosphate + H2O | - |
Clostridium perfringens | 4-methylumbelliferol + phosphate | - |
? | |
5'-AMP + H2O | - |
Clostridium perfringens | adenosine + phosphate | - |
? | |
5'-GMP + H2O | - |
Clostridium perfringens | guanosine + phosphate | - |
? | |
5'-TMP + H2O | - |
Clostridium perfringens | thymidine + phosphate | - |
? | |
5'-UMP + H2O | - |
Clostridium perfringens | uridine + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | further substrates: phenolphthalein bisphosphate, phenolphthalein monophosphate | Clostridium perfringens | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31000, analytical ultracentrifugation, gel filtration, SDS-PAGE | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
class C acid phosphatase | - |
Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
substrate p-nitrophenyl phosphate | Clostridium perfringens |
6 | - |
substrate 5'-UMP | Clostridium perfringens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
80% inhibition at 1 mM | Clostridium perfringens | Pb2+ | |
0.0012 | - |
- |
Clostridium perfringens | Hg2+ | |
0.5 | - |
- |
Clostridium perfringens | NaF |