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Literature summary for 3.1.3.2 extracted from

  • Panara, F.; Pasqualini, S.; Antonielli, M.
    Multiple forms of barley root acid phosphatase: purification and some characteristics of the major cytoplasmic isoenzyme (1990), Biochim. Biophys. Acta, 1037, 73-80.
    View publication on PubMed
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.25-10 mM, slight activation Hordeum vulgare
Triton X-100 0.05-1.5%, slight activation Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Hordeum vulgare
diethyl dicarbonate
-
 Hordeum vulgare
F- NaF Hordeum vulgare
Hg2+
-
 Hordeum vulgare
molybdate
-
 Hordeum vulgare
Pb2+
-
 Hordeum vulgare
phosphate competitive Hordeum vulgare
Urea 2.5 M, 50% inhibition Hordeum vulgare
Zn2+
-
 Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependence of Km Hordeum vulgare
0.12
-
 p-nitrophenyl phosphate pH 4.8 Hordeum vulgare
0.19
-
 p-nitrophenyl phosphate pH 4.2 Hordeum vulgare
0.32
-
 p-nitrophenyl phosphate pH 4.0 Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Hordeum vulgare 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Hordeum vulgare
77600
-
 gel filtration Hordeum vulgare
79000
-
 sucrose density gradient centrifugation Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 multiple enzyme forms
-

Purification (Commentary)

Purification (Comment) Organism
major cytoplasmic isoenzyme Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.7
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O 80% of the activity with p-nitrophenyl phosphate Hordeum vulgare 1-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O 105% of the activity with p-nitrophenyl phosphate Hordeum vulgare 2-naphthol + phosphate
-
 ?
ADP + H2O 20% of the activity with p-nitrophenyl phosphate Hordeum vulgare AMP + phosphate
-
 ?
AMP + H2O 18% of the activity with p-nitrophenyl phosphate Hordeum vulgare adenosine + phosphate
-
 ?
ATP + H2O 75% of the activity with p-nitrophenyl phosphate Hordeum vulgare ADP + phosphate
-
 ?
glucose 1-phosphate + H2O 33% of the activity with p-nitrophenyl phosphate Hordeum vulgare glucose + phosphate
-
 ?
O-phospho-L-Tyr + H2O 62% of the activity with p-nitrophenyl phosphate Hordeum vulgare Tyr + phosphate
-
 ?
O-phosphoserine + H2O 48% of the activity with p-nitrophenyl phosphate Hordeum vulgare Ser + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Hordeum vulgare p-nitrophenol + phosphate
-
 ?
phenyl phosphate + H2O 80% of the activity with p-nitrophenyl phosphate Hordeum vulgare phenol + phosphate
-
 ?
phosphothreonine + H2O 25% of the activity with p-nitrophenyl phosphate Hordeum vulgare Thr + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life: 40 min Hordeum vulgare
50
-
 half-life: 5 min Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 6.8
-
 Hordeum vulgare