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Literature summary for 3.1.3.2 extracted from
- Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer (2007), J. Biotechnol., 132, 82-87.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.434 | - |
p-nitrophenyl phosphate | estimated Km | Mucor hiemalis |  |
| 1.29 | - |
sodium phytate | estimated Km | Mucor hiemalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
native enzyme, gel filtration | Mucor hiemalis |
| 45000 | - |
purified enzyme, SDS-PAGE | Mucor hiemalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mucor hiemalis | - |
has phytase activity | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Mucor hiemalis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sepharose CL-6B column chromatography, Sephacryl S-100-HR gel filtration, phenyl agarose column chromatography, and Sephadex G-25 gel filtration | Mucor hiemalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
crude cell extract | Mucor hiemalis |
| 46.7 | - |
after 14.1fold purification | Mucor hiemalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl phosphate + H2O | - |
Mucor hiemalis | 1-naphthol + phosphate | - |
? | |
| 2-naphthyl phosphate + H2O | - |
Mucor hiemalis | 2-naphthol + phosphate | - |
? | |
| ADP + H2O | - |
Mucor hiemalis | AMP + phosphate | - |
? | |
| AMP + H2O | - |
Mucor hiemalis | adenosine + phosphate | - |
? | |
| ATP + H2O | - |
Mucor hiemalis | ADP + phosphate | - |
? | |
| calcium phytate + H2O | - |
Mucor hiemalis | ? | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Mucor hiemalis | D-glucose + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | - |
Mucor hiemalis | glycerol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Mucor hiemalis | p-nitrophenol + phosphate | - |
? | |
| phenyl phosphate + H2O | - |
Mucor hiemalis | phenol + phosphate | - |
? | |
| sodium phytate + H2O | - |
Mucor hiemalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Mucor hiemalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Mucor hiemalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
retains 64%, 63% and 61% of its original activity after heating for 1, 3 and 5 min, respectively, at 80°C | Mucor hiemalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Mucor hiemalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | retains almost 50% of optimal activity at pH 6.0, drops to 13% residual activity at pH 7.0 | Mucor hiemalis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mucor hiemalis | isoelectric focusing | - |
7.8 |
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