Literature summary for 3.1.3.2 extracted from
Patil, K.; Mahajan, R.; Lautre, H.; Patil, V.
Isolation, purification and characterization of acid phosphatase from Scenedesmus obliquus (2016), Res. J. Pharm. Biol. Chem. Sci., 7, 1584-1597 .
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
Ca2+ |
about 112% activity at 1 mM |
Tetradesmus obliquus |
|
Cu2+ |
about 130% activity at 1 mM |
Tetradesmus obliquus |
|
EDTA |
about 120% activity at 1 mM |
Tetradesmus obliquus |
|
Fe3+ |
about 120% activity at 1 mM |
Tetradesmus obliquus |
|
guaiacol |
about 120% activity at 1 mM |
Tetradesmus obliquus |
|
Triton X-100 |
about 115% activity at 1 mM |
Tetradesmus obliquus |
|
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
acetone |
about 70% residual activity at 1 mM |
Tetradesmus obliquus |
|
butanol |
about 40% residual activity at 1 mM |
Tetradesmus obliquus |
|
chloroform |
about 30% residual activity at 1 mM |
Tetradesmus obliquus |
|
hexane |
about 90% residual activity at 1 mM |
Tetradesmus obliquus |
|
Hg2+ |
about 95% residual activity at 1 mM |
Tetradesmus obliquus |
|
isoamyl alcohol |
about 80% residual activity at 1 mM |
Tetradesmus obliquus |
|
K+ |
strong inhibition, about 55% residual activity at 1 mM |
Tetradesmus obliquus |
|
Mg2+ |
about 95% residual activity at 1 mM |
Tetradesmus obliquus |
|
SDS |
strong inhibition, about 10% residual activity at 1 mM |
Tetradesmus obliquus |
|
Tween 80 |
strong inhibition, about 60% residual activity at 1 mM |
Tetradesmus obliquus |
|
Zn2+ |
about 70% residual activity at 1 mM |
Tetradesmus obliquus |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.24 |
- |
4-nitrophenyl phosphate |
at pH 5.0 and 37°C |
Tetradesmus obliquus |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
56100 |
- |
liquid crystal mass spectrometry analysis |
Tetradesmus obliquus |
Organism
Organism |
UniProt |
Comment |
Textmining |
Tetradesmus obliquus |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration |
Tetradesmus obliquus |
Storage Stability
Storage Stability |
Organism |
4°C, enzyme extracted with ammonium sulfate precipitation, 1 week, 13.8% loss of activity |
Tetradesmus obliquus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
4-nitrophenyl phosphate + H2O |
- |
Tetradesmus obliquus |
4-nitrophenol + phosphate |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
monomer |
1 * 59410, SDS-PAGE |
Tetradesmus obliquus |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
50 |
- |
- |
Tetradesmus obliquus |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
30 |
60 |
the enzyme is most active between 30°C and 60°C. It retains more than 44% activity till 60°C, while the activity drops rapidly below 30°C and above 60°C |
Tetradesmus obliquus |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
37 |
50 |
the enzyme is fairly stable for 120 min at temperature up to 37°C. Above 50°C, the activity declines rapidly as the temperature is increased |
Tetradesmus obliquus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5 |
- |
- |
Tetradesmus obliquus |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
4 |
6 |
the enzyme shows the most activity in the pH range 4.0-6.0, and its activity decreases sharply above pH 5.0 retaining 12% of maximum activity |
Tetradesmus obliquus |