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Literature summary for 3.1.3.2 extracted from
- Purification and characterization of a low molecular weight acid phosphatase from bovine liver (1969), J. Biol. Chem., 244, 299-307.
General Stability
| General Stability | Organism |
|---|---|
| phosphate and EDTA stabilize | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bos taurus |  |
| Mg2+ | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
p-nitrophenyl phosphate | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16590 | - |
equilibrium sedimentation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acid phosphatase III | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-UMP + H2O | 10% of the activity with p-nitrophenyl phosphate | Bos taurus | uridine + phosphate | - |
? | |
| FMN + H2O | 68% of the activity with p-nitrophenyl phosphate | Bos taurus | ? | - |
? | |
| galactose 6-phosphate + H2O | 39% of the activity with p-nitrophenyl phosphate | Bos taurus | galactose + phosphate | - |
? | |
| glucose 1-phosphate + H2O | 14% of the activity with p-nitrophenyl phosphate | Bos taurus | glucose + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Bos taurus | p-nitrophenol + phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | pH 5.0: about 65% of maximal activity, pH 6.0: about 75% of maximal activity | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | 60 h, 50% loss of activity | Bos taurus |
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