Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
I78A | mutant displays significantly higher (as high as 160%) enzymatic efficiency over a broad pH range of 3.0-9.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
I78D | mutant possesses nearly twice the specific activity at its optimum pH of 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
I78H | mutant exhibits an alkaline shift in the pH-optimum by 1.5 pH points relative to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.08 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.08 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.11 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.12 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.14 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.25 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.32 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.47 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.55 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.8 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | Q71EB8 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium MD6001 | Q71EB8 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, SP-Sepharose column chromatography, and phenyl-Sepharose column chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium MD6001 | p-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bNSAP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
class-A bacterial non-specific acid phosphatases | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
PhoN | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.99 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
2 | 8 | p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.5 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.8 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
5.9 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
8.1 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
11.83 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
12.1 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
16.1 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
16.9 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
18.24 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
19.7 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
19.95 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
20.84 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
21.53 | - |
p-nitrophenyl phosphate | mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
22.25 | - |
p-nitrophenyl phosphate | wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
23.32 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
26.42 | - |
p-nitrophenyl phosphate | mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
32.2 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
41.6 | - |
p-nitrophenyl phosphate | mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
wild type enzyme and mutant enzyme I78A | Salmonella enterica subsp. enterica serovar Typhimurium |
7 | - |
mutant enzymes I78D and I78H | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |