Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.2 extracted from

  • Yoneyama, T.; Taira, M.; Suzuki, T.; Nakamura, M.; Niwa, K.; Watanabe, T.; Ohyama, T.
    Expression and characterization of a recombinant unique acid phosphatase from kidney bean hypocotyl exhibiting chloroperoxidase activity in the yeast Pichia pastoris (2007), Protein Expr. Purif., 53, 31-39.
    View publication on PubMed
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA 104% activity at 0.1 mM Phaseolus vulgaris
additional information not affected by citrate Phaseolus vulgaris
NaF 114% activity at 0.01 mM Phaseolus vulgaris
Tartrate 110% activity at 0.1 mM Phaseolus vulgaris

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ 94% residual activity at 0.1 mM Phaseolus vulgaris
Mo6+ 13% residual activity at 0.1 mM Phaseolus vulgaris
VO43- 5% residual activity at 0.01 mM Phaseolus vulgaris
Zn2+ 70% residual activity at 0.1 mM Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 p-nitrophenyl phosphate wild type enzyme Phaseolus vulgaris
0.61
-
 p-nitrophenyl phosphate recombinant enzyme Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 108% activity at 0.01 mM Phaseolus vulgaris
Mn2+ 106% activity at 0.01 mM Phaseolus vulgaris
Zn2+ 101% activity at 0.01 mM Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme Phaseolus vulgaris
56000
-
 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme Phaseolus vulgaris
110000
-
 gel filtration, recombinant enzyme Phaseolus vulgaris
120000
-
 SDS-PAGE, recombinant enzyme Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
 variety kidney bean, cultivar Ohfuku
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Phaseolus vulgaris

Purification (Commentary)

Purification (Comment) Organism
ProBond resin nickel affinity column chromatography Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
 after 1fold purification, using p-nitrophenyl phosphate as a substrate Phaseolus vulgaris
32.2
-
 after 25fold purification, using p-nitrophenyl phosphate as a substrate Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phosphoglycerate + H2O 32% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris glycerol + phosphate
-
 ?
3-phosphoglycerate + H2O 34% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris glycerol + phosphate
-
 ?
ADP + H2O 54% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris AMP + phosphate
-
 ?
alpha-naphthylphosphate + H2O 32% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris alpha-naphthol + phosphate
-
 ?
AMP + H2O 13% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris adenosine + phosphate
-
 ?
ATP + H2O 40% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris ADP + phosphate
-
 ?
beta-naphthylphosphate + H2O 59% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris beta-naphthol + phosphate
-
 ?
D-glucose 1-phosphate + H2O 58% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 46% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris D-glucose + phosphate
-
 ?
diphosphate + H2O 65% activity compared to phosphoenolpyruvate Phaseolus vulgaris 2 phosphate
-
 ?
GTP + H2O 16% activity compared to phosphoenolpyruvate Phaseolus vulgaris GDP + phosphate
-
 ?
additional information the enzyme shows chloroperoxidase activity after the addition of orthovanadate, no activity with bis-p-nitrophenyl phosphate Phaseolus vulgaris ?
-
 ?
p-nitrophenyl phosphate + H2O 100% activity Phaseolus vulgaris p-nitrophenol + phosphate
-
 ?
phosphoenolpyruvate + H2O 67% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris pyruvate + phosphate
-
 ?
phosphothreonine + H2O 42% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris threonine + phosphate
-
 ?
phosphotyrosine + H2O 63% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris tyrosine + phosphate
-
 ?
phytate + H2O 16% activity compared to p-nitrophenyl phosphate Phaseolus vulgaris ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme Phaseolus vulgaris

Synonyms

Synonyms Comment Organism
KhACP shows also chloroperoxidase activity Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Phaseolus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4 7
-
 Phaseolus vulgaris