Cloned (Comment) | Organism |
---|---|
expressed in Arxula adeninivorans strain G1211 | Blastobotrys adeninivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
phytic acid | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
0.5 | - |
3-indoxyl phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
0.7 | - |
pyridoxal 5'-phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1.4 | - |
ADP | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1.4 | - |
AMP | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1.7 | - |
D-glucose 6-phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1.8 | - |
Sodium diphosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
2.5 | - |
p-nitrophenyl phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
2.5 | - |
1-naphthyl phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
2.5 | - |
Glycerol 2-phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52400 | - |
6 * 52400, calculated from amino acid sequence | Blastobotrys adeninivorans |
58330 | - |
6 * 58330, gel filtration | Blastobotrys adeninivorans |
314400 | - |
calculated from amino acid sequence | Blastobotrys adeninivorans |
350000 | - |
gel filtration | Blastobotrys adeninivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobotrys adeninivorans | - |
- |
- |
Blastobotrys adeninivorans LS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydroxylapatite column chromatography and Superdex 200 gel filtration | Blastobotrys adeninivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Blastobotrys adeninivorans | 1-naphthol + phosphate | - |
? | |
1-naphthyl phosphate + H2O | - |
Blastobotrys adeninivorans LS3 | 1-naphthol + phosphate | - |
? | |
3-indoxyl phosphate + H2O | - |
Blastobotrys adeninivorans | 3-indoxol + phosphate | - |
? | |
ADP + H2O | - |
Blastobotrys adeninivorans | AMP + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Blastobotrys adeninivorans | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Blastobotrys adeninivorans LS3 | D-glucose + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Blastobotrys adeninivorans | glycerol + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Blastobotrys adeninivorans LS3 | glycerol + phosphate | - |
? | |
additional information | no activity with cAMP | Blastobotrys adeninivorans | ? | - |
? | |
additional information | no activity with cAMP | Blastobotrys adeninivorans LS3 | ? | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Blastobotrys adeninivorans | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Blastobotrys adeninivorans LS3 | p-nitrophenol + phosphate | - |
? | |
phytic acid + H2O | - |
Blastobotrys adeninivorans | ? + phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | - |
Blastobotrys adeninivorans | pyridoxal + phosphate | - |
? | |
sodium diphosphate + H2O | - |
Blastobotrys adeninivorans | sodium phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 52400, calculated from amino acid sequence | Blastobotrys adeninivorans |
hexamer | 6 * 58330, gel filtration | Blastobotrys adeninivorans |
Synonyms | Comment | Organism |
---|---|---|
Apho1p | - |
Blastobotrys adeninivorans |
orthophosphoric-monoester phosphohydrolase | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Blastobotrys adeninivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | - |
Blastobotrys adeninivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
845 | - |
AMP | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
915 | - |
phytic acid | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1056 | - |
3-indoxyl phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1831 | - |
Glycerol 2-phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
1901 | - |
pyridoxal 5'-phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
2042 | - |
Sodium diphosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
2462 | - |
D-glucose 6-phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
2676 | - |
ADP | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
5282 | - |
1-naphthyl phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans | |
40850 | - |
p-nitrophenyl phosphate | recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C | Blastobotrys adeninivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Blastobotrys adeninivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.2 | 5.3 | - |
Blastobotrys adeninivorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Blastobotrys adeninivorans | isoelectric focusing | - |
5.13 |