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Literature summary for 3.1.3.2 extracted from

  • Dhatwalia, R.; Singh, H.; Reilly, T.J.; Tanner, J.J.
    Crystal structure and tartrate inhibition of Legionella pneumophila histidine acid phosphatase (2015), Arch. Biochem. Biophys., 585, 32-38 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(AI) cells Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with the inhibitor L(+)-tartrate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M sodium acetate at pH 4.5 Legionella pneumophila

Inhibitors

Inhibitors Comment Organism Structure
L-(+)-Tartrate
-
Legionella pneumophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
4-nitrophenyl phosphate at pH 5.5 and 37°C Legionella pneumophila
3.3
-
3'-AMP at pH 5.5 and 37°C Legionella pneumophila
3.9
-
5'-AMP at pH 5.5 and 37°C Legionella pneumophila

Organism

Organism UniProt Comment Textmining
Legionella pneumophila Q9APF7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and HiTrap Q column chromatography Legionella pneumophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
Legionella pneumophila adenosine + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Legionella pneumophila 4-nitrophenol + phosphate
-
?
5'-AMP + H2O
-
Legionella pneumophila adenosine + phosphate
-
?

Synonyms

Synonyms Comment Organism
HAP
-
Legionella pneumophila
histidine acid phosphatase
-
Legionella pneumophila
major acid phosphatase
-
Legionella pneumophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
5'-AMP at pH 5.5 and 37°C Legionella pneumophila
3
-
3'-AMP at pH 5.5 and 37°C Legionella pneumophila
16.1
-
4-nitrophenyl phosphate at pH 5.5 and 37°C Legionella pneumophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0043
-
L-(+)-Tartrate at pH 5.5 and 37°C Legionella pneumophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
5'-AMP at pH 5.5 and 37°C Legionella pneumophila
0.9
-
3'-AMP at pH 5.5 and 37°C Legionella pneumophila