Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(AI) cells | Legionella pneumophila |
Crystallization (Comment) | Organism |
---|---|
complexed with the inhibitor L(+)-tartrate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M sodium acetate at pH 4.5 | Legionella pneumophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-(+)-Tartrate | - |
Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
4-nitrophenyl phosphate | at pH 5.5 and 37°C | Legionella pneumophila | |
3.3 | - |
3'-AMP | at pH 5.5 and 37°C | Legionella pneumophila | |
3.9 | - |
5'-AMP | at pH 5.5 and 37°C | Legionella pneumophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | Q9APF7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and HiTrap Q column chromatography | Legionella pneumophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | - |
Legionella pneumophila | adenosine + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Legionella pneumophila | 4-nitrophenol + phosphate | - |
? | |
5'-AMP + H2O | - |
Legionella pneumophila | adenosine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAP | - |
Legionella pneumophila |
histidine acid phosphatase | - |
Legionella pneumophila |
major acid phosphatase | - |
Legionella pneumophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
5'-AMP | at pH 5.5 and 37°C | Legionella pneumophila | |
3 | - |
3'-AMP | at pH 5.5 and 37°C | Legionella pneumophila | |
16.1 | - |
4-nitrophenyl phosphate | at pH 5.5 and 37°C | Legionella pneumophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
L-(+)-Tartrate | at pH 5.5 and 37°C | Legionella pneumophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
5'-AMP | at pH 5.5 and 37°C | Legionella pneumophila | |
0.9 | - |
3'-AMP | at pH 5.5 and 37°C | Legionella pneumophila |