Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) RIPL cells | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
D73N | the mutation effaces RNase H activity but has virtually no effect on the acid phosphatase activity of the RnhC protein | Mycolicibacterium smegmatis |
H173A | the mutation does not affect RNase H activity but abolishes the acid phosphatase activity of the RnhC protein | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the acid phosphatase activity does not use a divalent cation cofactor | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His60-Ni Superflow resin column chromatography, Ni-NTA agarose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Mycolicibacterium smegmatis | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rnhC | bifunctional enzyme with RNase H (N-terminus) and acid phosphatase (C-terminus) activities | Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-nitrophenyl phosphate | at pH 5.5, temperature not specified in the publication | Mycolicibacterium smegmatis | |
0.33 | - |
4-nitrophenyl phosphate | at pH 4.5, temperature not specified in the publication | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Mycolicibacterium smegmatis |