Literature summary for 3.1.3.2 extracted from

Cox, R.S.; Schenk, G.; Mitic, N.; Gahan, L.R.; Hengge, A.C.
Diesterase activity and substrate binding in purple acid phosphatases (2007), J. Am. Chem. Soc., 129, 9550-9551.

Search for more literature for 3.1.3.2

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphate	-	Phaseolus vulgaris
phosphate	-	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.25	-	p-nitrophenyl phosphate	at pH 4.9	Sus scrofa
2.2	-	p-nitrophenyl phosphate	at pH 6.25	Phaseolus vulgaris
110	-	methyl-p-nitrophenyl phosphate	at pH 4.9	Sus scrofa
138	-	methylphosphate	at pH 4.9	Sus scrofa
281	-	methylphosphate	at pH 6.25	Phaseolus vulgaris
356	-	methyl-p-nitrophenyl phosphate	at pH 6.25	Phaseolus vulgaris

Organism

Organism	UniProt	Comment	Textmining
Phaseolus vulgaris	-	variety kidney bean	-
Sus scrofa	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl-p-nitrophenyl phosphate + H2O	-	Phaseolus vulgaris	p-nitrophenol + methyl phosphate	-	?
methyl-p-nitrophenyl phosphate + H2O	-	Sus scrofa	p-nitrophenol + methyl phosphate	-	?
methylphosphate + H2O	-	Phaseolus vulgaris	methanol + phosphate	-	?
methylphosphate + H2O	-	Sus scrofa	methanol + phosphate	-	?
p-nitrophenyl phosphate + H2O	-	Phaseolus vulgaris	p-nitrophenol + phosphate	-	?
p-nitrophenyl phosphate + H2O	-	Sus scrofa	p-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PAP	-	Phaseolus vulgaris
PAP	-	Sus scrofa
purple acid phosphatase	-	Phaseolus vulgaris
purple acid phosphatase	-	Sus scrofa
uteroferrin	-	Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17	-	methylphosphate	at pH 4.9	Sus scrofa
20	-	methylphosphate	at pH 6.25	Phaseolus vulgaris
470	-	p-nitrophenyl phosphate	at pH 4.9	Sus scrofa
542	-	methyl-p-nitrophenyl phosphate	at pH 4.9	Sus scrofa
542	-	methyl-p-nitrophenyl phosphate	at pH 6.25	Phaseolus vulgaris
850	-	p-nitrophenyl phosphate	at pH 6.25	Phaseolus vulgaris

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.9	-	-	Sus scrofa
6.25	-	-	Phaseolus vulgaris

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.8	-	phosphate	using methyl-p-nitrophenyl phosphate as a substrate, at pH 6.25	Phaseolus vulgaris
1.8	-	phosphate	using p-nitrophenyl phosphate as a substrate, at pH 6.25	Phaseolus vulgaris
4.1	-	phosphate	using methyl-p-nitrophenyl phosphate as a substrate, at pH 4.9	Sus scrofa

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Release 2023.1 (January 2023)