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Literature summary for 3.1.3.2 extracted from

  • Ou, Z.; Felts, R.L.; Reilly, T.J.; Nix, J.C.; Tanner, J.J.
    Crystallization of recombinant Haemophilus influenzae e (P4) acid phosphatase (2006), Acta Crystallogr. Sect. F, 62, 464-466.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with of 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.1-8.5 and 28-36% (w/v) PEG 4000 Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28510
-
full-length recombinant P4, mass spectrometry analysis Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Chelating HP column chromatography, Cu-affinity column chromatography, and HiTrap SP Sepharose column chromatography Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + H2O
-
Haemophilus influenzae pyruvate + phosphate
-
?

Synonyms

Synonyms Comment Organism
lipoprotein e class C acid phosphatase Haemophilus influenzae
P4
-
Haemophilus influenzae