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Literature summary for 3.1.3.2 extracted from

  • Chaimovich, H.; Nome, F.
    Purification and properties of an acid phosphatase from bovine brain (1970), Arch. Biochem. Biophys., 139, 9-16.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Bos taurus
Mn2+
-
 Bos taurus
NEM
-
 Bos taurus
p-Nitrophenyl sulfate inhibits hydrolysis of p-nitrophenyl phosphate Bos taurus
PCMB
-
 Bos taurus
phosphate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 p-nitrophenyl phosphate
-
 Bos taurus
0.075
-
 p-nitrophenyl phosphate
-
 Bos taurus
1
-
 alpha-glycerophosphate
-
 Bos taurus
1.4
-
 o-nitrophenyl phosphate
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O no activity Bos taurus AMP + phosphate
-
 ?
alpha-glycerophosphate + H2O 15% of the activity with p-nitrophenylphosphate Bos taurus glycerol + phosphate
-
 ?
AMP + H2O no activity Bos taurus adenosine + phosphate
-
 ?
D-fructose 6-phosphate + H2O no activity Bos taurus D-fructose + phosphate
-
 ?
dAMP + H2O 5% of the activity with p-nitrophenylphosphate Bos taurus deoxyadenosine + phosphate
-
 ?
diphosphate + H2O no activity Bos taurus 2 phosphate
-
 ?
glucose 1-phosphate + H2O no activity Bos taurus glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O no activity Bos taurus Glucose + phosphate
-
 ?
GMP + H2O no activity Bos taurus guanosine + phosphate
-
 ?
o-nitrophenyl phosphate + H2O 21% of the activity with p-nitrophenylphosphate Bos taurus o-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Bos taurus p-nitrophenol + phosphate
-
 ?
pyridoxal 5'-phosphate + H2O 5% of the activity with p-nitrophenylphosphate Bos taurus pyridoxal + phosphate
-
 ?
UMP + H2O 10% of the activity with p-nitrophenylphosphate Bos taurus uridine + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 10 min, pH 3.9-6.8, stable Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8 5.8
-
 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.9 6.8 37°C, 10 min, stable Bos taurus