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Literature summary for 3.1.3.2 extracted from

  • Rengasamy, A.; Selvam, R.; Gnanam, A.
    Isolation and properties of an acid phosphatase from thylakoid membranes of Sorghum vulgare (1981), Arch. Biochem. Biophys., 209, 230-236.
    View publication on PubMed
Search for more literature for 3.1.3.2

General Stability

General Stability Organism
5 M urea, 2 h, 71% of the initial activity is retained Sorghum bicolor
denaturation by guanidine hydrochloride and SDS Sorghum bicolor

Inhibitors

Inhibitors Comment Organism Structure
ATP competitive inhibitor of hydrolysis of p-nitrophenyl phosphate Sorghum bicolor
F-
-
 Sorghum bicolor
Hg2+ 5 mM HgCl2, 82% inhibition Sorghum bicolor
KH2PO4
-
 Sorghum bicolor
molybdate
-
 Sorghum bicolor
Pb(CH3COO)2
-
 Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 Phenyl phosphate
-
 Sorghum bicolor
0.3
-
 p-nitrophenyl phosphate
-
 Sorghum bicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid
-
 Sorghum bicolor 9579
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 1 * 26000, SDS-PAGE Sorghum bicolor
27000
-
 gel filtration Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-glycerate phosphate + H2O
-
 Sorghum bicolor glycerate + phosphate
-
 ?
2-naphthyl phosphate + H2O
-
 Sorghum bicolor 2-naphthol + phosphate
-
 ?
5'-ADP + H2O
-
 Sorghum bicolor AMP + phosphate
-
 ?
5'-GMP + H2O
-
 Sorghum bicolor guanosine + phosphate
-
 ?
ATP + H2O
-
 Sorghum bicolor ADP + phosphate
-
 ?
creatine phosphate + H2O
-
 Sorghum bicolor creatine + phosphate
-
 ?
D-fructose 1,6-diphosphate + H2O
-
 Sorghum bicolor ?
-
 ?
D-fructose 6-phosphate + H2O
-
 Sorghum bicolor D-fructose + phosphate
-
 ?
D-ribose 5-phosphate + H2O
-
 Sorghum bicolor D-ribose + phosphate
-
 ?
diphosphate + H2O
-
 Sorghum bicolor 2 phosphate
-
 ?
glucose 1-phosphate + H2O
-
 Sorghum bicolor glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O
-
 Sorghum bicolor Glucose + phosphate
-
 ?
GTP + H2O
-
 Sorghum bicolor GDP + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Sorghum bicolor p-nitrophenol + phosphate
-
 ?
phenyl phosphate + H2O
-
 Sorghum bicolor phenol + phosphate
-
 ?
phosphopyruvate + H2O
-
 Sorghum bicolor pyruvate + phosphate
-
 ?
UTP + 2 H2O
-
 Sorghum bicolor UMP + 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Sorghum bicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 30% loss of activity Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Sorghum bicolor