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Literature summary for 3.1.3.2 extracted from

  • Wang, R.; Ohtani, K.; Wang, Y.; Yuan, Y.; Hassan, S.; Shimizu, T.
    Genetic and biochemical analysis of a class C non-specific acid phosphatase (NSAP) of Clostridium perfringens (2010), Microbiology, 156, 167-173.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100
-
Clostridium perfringens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-fusion protein Clostridium perfringens

Inhibitors

Inhibitors Comment Organism Structure
SDS
-
Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.84
-
4-nitrophenyl phosphate pH 4.8, 37°C Clostridium perfringens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM Mg2Cl2 enhances activity Clostridium perfringens
NaF inhibits activity Clostridium perfringens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
calculated from cDNA Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Clostridium perfringens 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
acid phosphatase
-
Clostridium perfringens
CPE0201
-
Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium perfringens
40
-
optimal activity Clostridium perfringens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
activity drops rapidly by 78% at 50°C and by 84% at 55°C Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Clostridium perfringens

pH Range

pH Minimum pH Maximum Comment Organism
4.6 5.2 enzyme retains only 27% of the optimal activity at pH 4.4, which is rapidly increased to 60% at pH 4.6. It retains 94% of optimal activity at pH 5.0, but the activity dramatically drops to 67% at pH 5.2 Clostridium perfringens

General Information

General Information Comment Organism
malfunction A CPE0201 mutant does not grow in a minimal medium containing 4-nitrophenyl phosphate, while it shows normal growth when Na2HPO4 is added to the medium Clostridium perfringens
physiological function enzyme is associated with the surface of the cell, where it may function to acquire inorganic phosphate from organic phosphomonoesters in acidic conditions, which could play an important role in the survival and growth of Clostridium perfringens in the host tissue Clostridium perfringens