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Literature summary for 3.1.3.2 extracted from

  • Szalewicz, A.; Strzelczyk, B.; Sopel, M.; Kubicz, A.
    The 35 kDa acid metallophosphatase of the frog Rana esculenta liver: studies on its cellular localization and protein phosphatase activity (2003), Acta Biochim. Pol., 50, 555-566.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
molybdate strong competitive inhibitor, competitive with 4-nitrophenyl phosphate Pelophylax lessonae
additional information the enzyme is not inhibited by okadaic acid and F- Pelophylax lessonae
phosphate competitive with 4-nitrophenyl phosphate, competitive with 4-nitrophenyl phosphate Pelophylax lessonae
phosphotyrosine competitive with 4-nitrophenyl phosphate Pelophylax lessonae
tungstate strong competitive inhibitor, competitive with 4-nitrophenyl phosphate Pelophylax lessonae
vanadate strong competitive inhibitor, competitive with 4-nitrophenyl phosphate Pelophylax lessonae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00755
-
hirudin 53-65 C-terminal fragment pH 5.0, 37°C Pelophylax lessonae
0.273
-
phosvitin per number of phosphoserine residues, pH 5.0, 37°C Pelophylax lessonae
1.38
-
phosphotyrosine pH 5.0, 37°C Pelophylax lessonae
1.39
-
4-nitrophenyl phosphate pH 5.0, 37°C Pelophylax lessonae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pelophylax lessonae 5737
-

Organism

Organism UniProt Comment Textmining
Pelophylax lessonae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte enzyme does not appear in other cells of liver tissue Pelophylax lessonae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Pelophylax lessonae 4-nitrophenol + phosphate
-
?
hirudin 53-65 C-terminal fragment + H2O containing phosphotyrosine63 Pelophylax lessonae ?
-
?
phosphotyrosine + H2O
-
Pelophylax lessonae phosphate + tyrosine
-
?
phosvitin + H2O protein containing phosphoserine, poor substrate Pelophylax lessonae ?
-
?
rabbit 32P-phosphorylase a + H2O protein containing phosphoserine, poor substrate Pelophylax lessonae ?
-
?

Synonyms

Synonyms Comment Organism
LMW AcPase
-
Pelophylax lessonae
low molecular mass acid phosphatase
-
Pelophylax lessonae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000036
-
tungstate pH 5.0, 37°C Pelophylax lessonae
0.00055
-
molybdate pH 5.0, 37°C Pelophylax lessonae
0.00955
-
vanadate pH 5.0, 37°C Pelophylax lessonae
0.213
-
phosphate pH 5.0, 37°C Pelophylax lessonae