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Literature summary for 3.1.3.2 extracted from
- Structure of recombinant Haemophilus influenzae e (P4) acid phosphatase reveals a new member of the haloacid dehalogenase superfamily (2007), Biochemistry, 46, 11110-11119.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Haemophilus influenzae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapour diffusion method with 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.1-8.5, and 28-36% (w/v) PEG 4000 | Haemophilus influenzae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F48C | reduced phosphatase activity | Haemophilus influenzae |
| N218Q | reduced phosphatase activity | Haemophilus influenzae |
| Q39E | reduced phosphatase activity | Haemophilus influenzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tungstate | - |
Haemophilus influenzae |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | metalloenzyme with Mg2+ being the most likely metal ion in the crystalline recombinant enzyme | Haemophilus influenzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Haemophilus influenzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl phosphate + H2O | - |
Haemophilus influenzae | 4-methylumbelliferol + phosphate | - |
? | |
| nicotinamide mononucleotide + H2O | - |
Haemophilus influenzae | nicotinamide riboside + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Haemophilus influenzae | p-nitrophenol + phosphate | - |
? | |
| phenyl phosphate + H2O | - |
Haemophilus influenzae | phenol + phosphate | - |
? | |
| phosphotyrosine + H2O | - |
Haemophilus influenzae | tyrosine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x-ray crystallography | Haemophilus influenzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipoprotein e acid phosphatase | - |
Haemophilus influenzae |
| P4 | - |
Haemophilus influenzae |
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Release 2023.1 (January 2023)
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