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Literature summary for 3.1.3.2 extracted from

  • Lau, K.H.W.; Farley, J.R.; Baylink, D.J.
    Bone acid phosphatase is a neutral pH phosphoryl protein phosphatase (1987), Adv. Protein Phosphatases, 4, 165-198.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Cys activates Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Bos taurus
Co2+ competitive Bos taurus
diphosphate
-
Bos taurus
F- competitive Bos taurus
molybdate competitive Bos taurus
phosphate competitive Bos taurus
Tartrate tartrate has no effect Bos taurus
vanadate competitive Bos taurus
Zn2+ above 1 mM, uncompetitive Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
DL-phosphotyrosine pH 7 Bos taurus
0.014
-
p-nitrophenyl phosphate pH 7 Bos taurus
0.064
-
p-nitrophenyl phosphate pH 8 Bos taurus
0.083
-
DL-phosphotyrosine pH 8 Bos taurus
0.44
-
p-nitrophenyl phosphate pH 9 Bos taurus
0.45
-
DL-phosphotyrosine pH 8 Bos taurus
1.37
-
DL-phosphotyrosine pH 9 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Bos taurus
Mn2+ activates Bos taurus
Zn2+ activates below 1 mM Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
1 * 32500, SDS-PAGE Bos taurus
34600
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate formation of a covalent phosphoryl-enzyme intermediate, kinetic pattern of a pseudo uni-bi hydrolytic two-step reaction mechanism Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.15
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O at 12.9% of the activity with p-nitrophenyl phosphate Bos taurus AMP + phosphate
-
?
ATP + H2O at 18.0% of the activity with p-nitrophenyl phosphate Bos taurus ADP + phosphate
-
?
CDP + H2O at 6.6% of the activity with p-nitrophenyl phosphate Bos taurus CMP + phosphate
-
?
DL-phosphotyrosine + H2O
-
Bos taurus tyrosine + phosphate
-
?
GDP + H2O at 18.5% of the activity with p-nitrophenyl phosphate Bos taurus GMP + phosphate
-
?
GTP + H2O at 15.3% of the activity with p-nitrophenyl phosphate Bos taurus GDP + phosphate
-
?
O-phospho-L-Tyr + H2O at 8.2% of the activity with p-nitrophenyl phosphate Bos taurus Tyr + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Bos taurus p-nitrophenol + phosphate
-
?
phosphotyrosyl histone + H2O 4.2% of the activity with p-nitrophenyl phosphate Bos taurus histone + phosphate
-
?
TDP + H2O at 10.6% of the activity with p-nitrophenyl phosphate Bos taurus TMP + phosphate
-
?
TTP + H2O at 11.3% of the activity with p-nitrophenyl phosphate Bos taurus TDP + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32500, SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
30 min, slight inactivation Bos taurus
90
-
rapid and irreversible inactivation Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
hydrolysis of p-nitrophenyl phosphate Bos taurus