Literature summary for 3.1.3.2 extracted from

Leitao, V.O.; de Melo Lima, R.C.; Vainstein, M.H.; Ulhoa, C.J.
Purification and characterization of an acid phosphatase from Trichoderma harzianum (2010), Biotechnol. Lett., 32, 1083-1088.

Search for more literature for 3.1.3.2

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibited by tartrate, EDTA, EGTA	Trichoderma harzianum
phosphate	partially inhibited	Trichoderma harzianum
sodium tungstate	strongly inhibited	Trichoderma harzianum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000165	-	p-nitrophenyl phosphate	-	Trichoderma harzianum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	x * 58000, SDS-PAGE	Trichoderma harzianum

Organism

Organism	UniProt	Comment	Textmining
Trichoderma harzianum	-	-	-
Trichoderma harzianum ALL42	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hydrophobic interaction chromatography (phenyl-Sepharose)	Trichoderma harzianum

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture filtrate	-	Trichoderma harzianum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
12.4	-	-	Trichoderma harzianum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl phosphate + H2O	further substrates: AMP, ADP, ATP, phenyl phosphate, fructose 6-phosphate and glucose 1-phosphate	Trichoderma harzianum	p-nitrophenol + phosphate	-	?
p-nitrophenyl phosphate + H2O	further substrates: AMP, ADP, ATP, phenyl phosphate, fructose 6-phosphate and glucose 1-phosphate	Trichoderma harzianum ALL42	p-nitrophenol + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 58000, SDS-PAGE	Trichoderma harzianum

Synonyms

Synonyms	Comment	Organism
acid phosphatase	-	Trichoderma harzianum
orthophosphoric-monoester phosphohydrolase	-	Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	at pH 4.8	Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	45°C, pH5.0, 60 min: 86% activity, 50°C, pH5.0, 60 min: 67% activity, 55°C, pH4.8, 60 min: 67% activity	Trichoderma harzianum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.8	-	-	Trichoderma harzianum

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Release 2023.1 (January 2023)