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Literature summary for 3.1.3.2 extracted from
- Partial purification and characterization of thermostable acid phosphatase from thermoacidophilic archeon Sulfolobus acidocaldarius (2000), Curr. Microbiol., 40, 57-60.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
p-nitrophenyl phosphate | - |
Sulfolobus acidocaldarius |  |
| 1.9 | - |
4-nitrophenyl phosphate | pH 5.0, 50°C | Sulfolobus acidocaldarius | |
| 1.9 | - |
p-nitrophenyl phosphate | in 50 mM Na-acetate (pH 5.0), at 50°C | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration | Sulfolobus acidocaldarius |
| 20000 | - |
about 20000 Da, gel filtration | Sulfolobus acidocaldarius |
| 25000 | - |
SDS-PAGE | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
| Sulfolobus acidocaldarius | - |
strain ATCC33909T | - |
| Sulfolobus acidocaldarius ATCC 33909T | - |
strain ATCC33909T | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephadex A-25 gel filtration and Superdex 75 gel filtration | Sulfolobus acidocaldarius |
| partial | Sulfolobus acidocaldarius |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| with 6 M guanidine-HCl at 25°C | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Sulfolobus acidocaldarius | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Sulfolobus acidocaldarius ATCC 33909T | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 25000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: about 40% of maximal activity, 80°C: about 60% of maximal activity | Sulfolobus acidocaldarius |
| 50 | 80 | 50°C: about 45% of maximal activity, 80°C: about 65% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, about 10% loss of activity | Sulfolobus acidocaldarius |
| 75 | - |
60 min, 40% loss of activity | Sulfolobus acidocaldarius |
| 75 | - |
60% activity remains after 60 min of heat treatment at 75°C | Sulfolobus acidocaldarius |
| 100 | - |
30 min, complete inactivation | Sulfolobus acidocaldarius |
| 100 | - |
the enzyme loses activity rapidly at 100°C for 30 min | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | pH 3.0: about 60% of maximal activity, pH 6.0: about 40% of maximal activity | Sulfolobus acidocaldarius |
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