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Literature summary for 3.1.3.2 extracted from
- Identification and characterization of the acid phosphatase HppA in Helicobacter pylori (2011), J. Microbiol. Biotechnol., 21, 483-493.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits enzymatic activity | Helicobacter pylori |  |
| Mn2+ | inhibits enzymatic activity | Helicobacter pylori | |
| Zn2+ | inhibits enzymatic activity | Helicobacter pylori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
4-nitrophenyl phosphate | pH 6.0, 37°C, periplasmic enzyme with 0.1 mM NiCl2 | Helicobacter pylori | |
| 0.43 | - |
4-nitrophenyl phosphate | pH 6.0, 37°C, periplasmic enzyme without Ni2+ | Helicobacter pylori | |
| 0.67 | - |
4-nitrophenyl phosphate | pH 6.0, 37°C, periplasmic enzyme with 0.1 mM NiCl2 | Helicobacter pylori | |
| 0.76 | - |
4-nitrophenyl phosphate | pH 6.0, 37°C, membrane-bound enzyme | Helicobacter pylori | |
| 0.79 | - |
ATP | pH 6.0, 37°C, membrane-bound enzyme | Helicobacter pylori | |
| 4.14 | - |
4-nitrophenyl phosphate | pH 6.0, 37°C, periplasmic enzyme without Ni2+ | Helicobacter pylori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Helicobacter pylori | 16020 | - |
| periplasm | - |
Helicobacter pylori | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | under acidic conditions (pH below 4.5) periplasmic enzymatic activity is dependent on the presence of mono- and/or divalent cations. Hydrolsis of 4-nitrophenyl phosphate is enhanced but not hydrolsis of ATP | Helicobacter pylori | |
| NH4+ | under acidic conditions (pH below 4.5) periplasmic enzymatic activity is dependent on the presence of mono- and/or divalent cations | Helicobacter pylori | |
| Ni2+ | under acidic conditions (pH below 4.5) periplasmic enzymatic activity is dependent on the presence of mono- and/or divalent cations. Ni2+ lowers Km without changing Vmax | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
SDS-PAGE, periplasmic fraction | Helicobacter pylori |
| 27000 | - |
SDS-PAGE, membrane-bound form | Helicobacter pylori |
| 72000 | - |
trimeric native from of the enzyme | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 192- and 34fold from the periplasmic and membrane fractions of Helicobacter pylori. Purification steps of membrane enzyme: Solubilization, DEAE-Sepharose and Phe-Sepharose. Purification steps of periplasmic enzyme: (NH4)2SO4 precipitation, SP-Sepharose, Phe-Sepharose and Q-Sepharose | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Helicobacter pylori | 4-nitrophenol + phosphate | - |
? | |
| ATP + H2O | periplasmic enzyme: stronger hydrolysis at pH 5.5 than at pH 6.0 | Helicobacter pylori | ADP + phosphate | - |
? | |
| CTP + H2O | periplasmic enzyme: stronger hydrolysis at pH 6.0 than at pH 5.5 | Helicobacter pylori | CDP + phosphate | - |
? | |
| TTP + H2O | periplasmic enzyme: stronger hydrolysis at pH 6.0 than at pH 5.5 | Helicobacter pylori | TDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HppA | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
periplasmic enzyme: adenosine nucleotides are better hydrolyzed at pH 5.5 than at pH 6.0 | Helicobacter pylori |
| 6 | - |
assay at | Helicobacter pylori |
| 6 | - |
periplasmic enzyme: CTP and TTP are better hydrolyzed at pH 6 than at pH 5.5 | Helicobacter pylori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
periplasmic enzyme is inactive at pH below 4.5 but displays activity in the presence of NH4Cl and NiCl2 | Helicobacter pylori |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | HppA knockout cells grow more slowly. Knockout cells import Mg2+ at a markedly lowered rate, but assimilate urea rapidly, with a subsequent increase in extracellular pH. Moreover, mutant cells are much more sensitive to extracellular potassium ions, as well as to metronidazole, omeprazole, or thiophenol, with considerably lowered MIC values, than wild-type cells | Helicobacter pylori |
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