Literature summary for 3.1.3.2 extracted from

Zhou, D.; Pan, Y.; Chen, X.; Zhang, N.; Ge, H.
Crystallization and preliminary crystallographic analysis of the major acid phosphatase from Legionella pneumophila (2015), Acta Crystallogr. Sect. F, 71, 779-783 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta cells	Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo-enzyme or in complex with L-(+)-tartrate, hanging drop vapor diffusion method, using 0.2 M sodium formate, 17% m(w/v) PEG 3350, 0.025% (w/v) low gelling temperature agarose, or 0.1 M Tris pH 8.5, 21% (w/v) PEG 6000, 0.025% (w/v) low gelling temperature agarose, respectively	Legionella pneumophila

Inhibitors

Inhibitors	Comment	Organism	Structure
L-(+)-Tartrate	-	Legionella pneumophila

Organism

Organism	UniProt	Comment	Textmining
Legionella pneumophila	Q5ZWG6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Legionella pneumophila

Subunits

Subunits	Comment	Organism
?	x * 39500, calculated from amino acid sequence	Legionella pneumophila

Synonyms

Synonyms	Comment	Organism
major acid phosphatase	-	Legionella pneumophila
MAP	-	Legionella pneumophila

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Release 2023.1 (January 2023)